Hydrogenology
Hydrogenology editorial study record

Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release

Liu Y et al. · BMC Gastroenterol. 2014;14:12.

PreclinicalOther formsPublished 2014
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Controlled rat partial-warm-liver-ischemia-reperfusion dose-response and time-course experiment

Research topic

Liver health

Administration classification

Other forms

Study result signal

Not reported in this record.

Outcome type

Not reported in this record.

Reported in the source

Methods at a glance

Population or model

Male Sprague-Dawley rats undergoing 60 minutes of 70% partial liver ischemia

Sample

Principal dose-response comparisons used n=8/group; time-course and molecular assays commonly used n=6–8/group

Duration

Single prereperfusion injection; assessments at 2, 6, 12 and 24 hours

Intervention

Hydrogen-enriched saline 2.5, 5 or 10 mL/kg intraperitoneally 10 minutes before reperfusion

Hydrogen form

H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.

H₂ specification

The article labels the intervention as 1% hydrogen-enriched saline; doses were 2.5–10 mL/kg.

H₂ flow

Not applicable — intraperitoneal saline, not gas inhalation.

O₂ delivered with H₂

No O₂ was co-delivered as part of the H₂ intervention.

Comparator

Ischemia-reperfusion with ordinary saline, injury without injection and sham surgery

Reported, not endorsed

Outcomes and reported result

Outcomes measured

ALT, histology, MDA, HNE, 8-OH-G, HMGB1, TNF-α and IL-6

Reported result

The article reports lower ALT, histologic injury, oxidative markers, HMGB1 and inflammatory cytokines with H₂-enriched saline.

Results-extraction completeness

Allocation, dose response, time course, principal figures, funding and conflicts checked in the open-access full text.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Acute rat ischemia-reperfusion model with several doses, time points and biochemical outcomes.

Applies directly to

Warm partial-liver ischemia-reperfusion in rats, not liver surgery or transplantation outcomes in people.

Preliminary appraisal framework

Not reported in this record.

Preliminary risk-of-bias status

Not reported in this record.

Appraisal rationale

Not reported in this record.

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 24410860 · DOI: 10.1186/1471-230X-14-12

Publisher access

Open-access full text is available through PMC and the publisher.

Extraction basis

Open-access PMC/publisher full text and PubMed record; no retraction or expression of concern was shown in the checked records on 7 August 2026.

Record revision

2026-08-10

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