Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release
Liu Y et al. · BMC Gastroenterol. 2014;14:12.
Study at a glance
Preclinical
Male Sprague-Dawley rats undergoing 60 minutes of 70% partial liver ischemia
Hydrogen-enriched saline 2.5, 5 or 10 mL/kg intraperitoneally 10 minutes before reperfusion · The article labels the intervention as 1% hydrogen-enriched saline; doses were 2.5–10 mL/kg.
Single prereperfusion injection; assessments at 2, 6, 12 and 24 hours
The article reports lower ALT, histologic injury, oxidative markers, HMGB1 and inflammatory cytokines with H₂-enriched saline.
Acute rat ischemia-reperfusion model with several doses, time points and biochemical outcomes.
What kind of evidence is this?
Preclinical
Controlled rat partial-warm-liver-ischemia-reperfusion dose-response and time-course experiment
Liver health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male Sprague-Dawley rats undergoing 60 minutes of 70% partial liver ischemia
Principal dose-response comparisons used n=8/group; time-course and molecular assays commonly used n=6–8/group
Single prereperfusion injection; assessments at 2, 6, 12 and 24 hours
Hydrogen-enriched saline 2.5, 5 or 10 mL/kg intraperitoneally 10 minutes before reperfusion
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
The article labels the intervention as 1% hydrogen-enriched saline; doses were 2.5–10 mL/kg.
Ischemia-reperfusion with ordinary saline, injury without injection and sham surgery
Outcomes and reported result
ALT, histology, MDA, HNE, 8-OH-G, HMGB1, TNF-α and IL-6
The article reports lower ALT, histologic injury, oxidative markers, HMGB1 and inflammatory cytokines with H₂-enriched saline.
Allocation, dose response, time course, principal figures, funding and conflicts checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Acute rat ischemia-reperfusion model with several doses, time points and biochemical outcomes.
Warm partial-liver ischemia-reperfusion in rats, not liver surgery or transplantation outcomes in people.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 24410860 · DOI: 10.1186/1471-230X-14-12
Open-access full text is available through PMC and the publisher.
Open-access PMC/publisher full text and PubMed record; no retraction or expression of concern was shown in the checked records on 7 August 2026.
10 August 2026
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