Molecular hydrogen protects against ischemia-reperfusion injury in a mouse fatty-liver model via regulating HO-1 and Sirt1 expression
Li S et al. · Sci Rep. 2018;8:14019.
Study at a glance
Preclinical
Mice with diet-induced fatty liver and isolated hepatocytes and Kupffer cells
Hydrogen-rich saline containing 7 ppm H₂ during ischemia-reperfusion; cell experiments used H₂-treated culture conditions · 7 ppm dissolved H₂ in saline for the in-vivo intervention.
15 minutes of ischemia and 3 hours of reperfusion in the principal animal experiment
The article reports less liver injury, macrophage activation, inflammation and apoptosis and higher HO-1 and Sirt1-related measures with H₂ treatment.
Preclinical fatty-liver model with short reperfusion, small groups and separate cell experiments. Several authors were affiliated with the company MiZ.
What kind of evidence is this?
Preclinical
Controlled mouse fatty-liver ischemia-reperfusion experiment plus primary-cell hypoxia-reoxygenation experiments
Liver health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Mice with diet-induced fatty liver and isolated hepatocytes and Kupffer cells
Principal animal figures report 5–7 mice per group; cell experiments used triplicate independent comparisons
15 minutes of ischemia and 3 hours of reperfusion in the principal animal experiment
Hydrogen-rich saline containing 7 ppm H₂ during ischemia-reperfusion; cell experiments used H₂-treated culture conditions
H₂ dissolved in saline for the animal intervention and H₂-treated medium for cells — not Brown’s gas and not inhalation by the animals.
7 ppm dissolved H₂ in saline for the in-vivo intervention.
Fatty-liver ischemia-reperfusion without H₂ and corresponding cell controls
Outcomes and reported result
Necrosis, macrophage infiltration, oxidative stress, apoptosis, AST, ALT, cytokines, HO-1 and Sirt1 signaling
The article reports less liver injury, macrophage activation, inflammation and apoptosis and higher HO-1 and Sirt1-related measures with H₂ treatment.
Concentration, main methods, figures, conflicts and principal results checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical fatty-liver model with short reperfusion, small groups and separate cell experiments. Several authors were affiliated with the company MiZ.
Diet-induced fatty liver ischemia-reperfusion in mice and isolated cells, not human liver surgery.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 30232347 · DOI: 10.1038/s41598-018-32411-4
Open-access full text is available through PMC and Scientific Reports.
Open-access PMC/publisher full text and PubMed record.
10 August 2026
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