Hydrogen suppresses oxidative stress by inhibiting the p38 MAPK signaling pathway in preeclampsia
Guo L, Liu M, Duan T. · Adv Clin Exp Med. 2023;32(3):357–367.
Study at a glance
Preclinical
Pregnant Sprague-Dawley rats in an L-NAME preeclampsia model.
Hydrogen-rich saline in the L-NAME intervention arms, with a p38 inhibitor in one additional arm. · L-NAME was delivered in hydrogen-rich saline; the full article describes preparation and arm-specific dosing.
Late-gestation experimental protocol.
The hydrogen-rich-saline arm had lower proteinuria and several placental oxidative, inflammatory and p38-pathway measures than the L-NAME model arm.
Animal-model evidence only; the inhibitor arm is a mechanistic co-intervention and does not establish clinical benefit.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Women’s health
Other forms
Positive preclinical signal with mechanistic co-intervention.
Physiological, tissue and biomarker outcomes in a rat model.
Methods
Pregnant Sprague-Dawley rats in an L-NAME preeclampsia model.
Five experimental groups; group sizes were checked in the full methods.
Late-gestation experimental protocol.
Hydrogen-rich saline in the L-NAME intervention arms, with a p38 inhibitor in one additional arm.
H₂ dissolved in saline.
L-NAME was delivered in hydrogen-rich saline; the full article describes preparation and arm-specific dosing.
Non-pregnant, normal-pregnancy and L-NAME saline controls.
Outcomes and reported result
Blood pressure, proteinuria, placental oxidative and inflammatory markers and p38 MAPK expression.
The hydrogen-rich-saline arm had lower proteinuria and several placental oxidative, inflammatory and p38-pathway measures than the L-NAME model arm.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal-model evidence only; the inhibitor arm is a mechanistic co-intervention and does not establish clinical benefit.
The reported L-NAME rat model only.
SYRCLE animal-study tool — preliminary
Some concerns
Randomization was reported, but concealment and assessor blinding were not established and several outcomes were analyzed.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36330842 · DOI: 10.17219/acem/154623
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
Help people check the facts before they buy.
Hydrogenology keeps 665 source-checked records accessible without advertising or product promotion.
Help us correct the recordReport a discrepancy
Send the exact difference between this page and the linked source. The study reference is attached automatically.