Hydrogenology
Hydrogenology editorial study record

Silicon-based agent mitigates fatty liver formation in a CDAHFD60-induced MASH mouse model by enhancing hepatic function.

Koyama Y, Kobayashi Y, Hirota I, Kobayashi H, Shimada S. · Biochemistry and Biophysics Reports. 2026;46:102552.

PreclinicalOther formsPublished 2026
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Randomized controlled mouse study

Research topic

Metabolic and lipid health

Administration classification

Other forms

Study result signal

Mixed positive preclinical signal.

Outcome type

Tissue, histological and biomarker outcomes in an animal model.

Reported in the source

Methods at a glance

Population or model

Male C57BL/6J mice fed a CDAHFD60 diet to model metabolic dysfunction-associated steatohepatitis.

Sample

26 mice; 13 per diet group after a two-week induction period.

Duration

12 weeks of intervention after two weeks of model induction.

Intervention

CDAHFD60 diet containing 2.5% of a silicon-based hydrogen-generating agent.

Hydrogen form

Hydrogen generated in the gastrointestinal tract by a silicon-based dietary agent; not Brown's gas.

H₂ specification

The study evaluated a hydrogen-generating material rather than a measured dose of dissolved or inhaled H2.

H₂ flow

Not applicable — dietary agent.

O₂ delivered with H₂

No oxygen was co-delivered.

Comparator

The same CDAHFD60 diet without the silicon-based agent.

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Liver histology, hepatic and serum lipids, oxidative-stress measures and biochemical markers.

Reported result

The supplemented group had less hepatic steatosis and favorable changes in several lipid and oxidative-stress measures; not every measured endpoint differed between groups.

Results-extraction completeness

The full article, methods, figures and positive and null findings were checked.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Animal evidence only. The intervention is a hydrogen-generating silicon product, so effects cannot automatically be assigned to molecular hydrogen alone.

Applies directly to

The reported CDAHFD60 mouse model only.

Preliminary appraisal framework

SYRCLE animal-study tool — preliminary

Preliminary risk-of-bias status

Some concerns

Appraisal rationale

Small animal study with reported randomization, but allocation concealment and assessor blinding were not established from the article.

Appraisal domains

Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 41890216 · DOI: 10.1016/j.bbrep.2026.102552

Publisher access

Free full article in PubMed Central.

Extraction basis

PubMed record and PubMed Central full text; extraction checked 9 August 2026.

Record revision

2026-08-10