Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of PARP-1 in retinal ischemia-reperfusion injury
Liu Y et al. · Mol Med Rep. 2015;12(2):2495–2502.
What kind of evidence is this?
Preclinical
Controlled rat retinal ischemia-reperfusion experiment
Eye health
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
Rats with retinal ischemia-reperfusion injury induced by transient elevation of intraocular pressure
Group-level histology assays commonly report n=6; the total number of animals was not clearly stated in the extracted article text
Daily treatment from reperfusion until the scheduled tissue assessments
Hydrogen-rich saline, 5 mL/kg intraperitoneally once daily from reperfusion until sacrifice
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
The introduction discusses theoretical saturation up to 0.8 mM; the actual preparation concentration was not clearly stated in the extracted methods.
Not applicable — intraperitoneal saline, not gas inhalation.
No O₂ was co-delivered as part of the intervention.
Ischemia-reperfusion animals receiving non-hydrogen saline, with sham-operated controls
Outcomes and reported result
Retinal morphology, cell loss, TUNEL staining, 8-OHdG, PARP-1 and caspase-3
The article reports less retinal cell loss, DNA oxidative-stress signal, PARP-1 overactivation and caspase-3 activation with hydrogen-rich saline.
Methods and principal laboratory outcomes checked in the free full text; exact total animal allocation and actual saline H₂ concentration remain unclear.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Animal injury model with laboratory and histologic outcomes; total animal allocation and actual H₂ concentration require further clarification. It cannot establish benefit in people.
Experimental retinal ischemia-reperfusion injury in rats, not human eye disease.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 25954991 · DOI: 10.3892/mmr.2015.3731
Open-access full text is available through PMC.
Open-access PMC full text and PubMed bibliographic record.
2026-08-10