Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of PARP-1 in retinal ischemia-reperfusion injury
Liu Y et al. · Mol Med Rep. 2015;12(2):2495–2502.
Study at a glance
Preclinical
Rats with retinal ischemia-reperfusion injury induced by transient elevation of intraocular pressure
Hydrogen-rich saline, 5 mL/kg intraperitoneally once daily from reperfusion until sacrifice · The introduction discusses theoretical saturation up to 0.8 mM; the actual preparation concentration was not clearly stated in the extracted methods.
Daily treatment from reperfusion until the scheduled tissue assessments
The article reports less retinal cell loss, DNA oxidative-stress signal, PARP-1 overactivation and caspase-3 activation with hydrogen-rich saline.
Animal injury model with laboratory and histologic outcomes; total animal allocation and actual H₂ concentration require further clarification. It cannot establish benefit in people.
What kind of evidence is this?
Preclinical
Controlled rat retinal ischemia-reperfusion experiment
Eye health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Rats with retinal ischemia-reperfusion injury induced by transient elevation of intraocular pressure
Group-level histology assays commonly report n=6; the total number of animals was not clearly stated in the extracted article text
Daily treatment from reperfusion until the scheduled tissue assessments
Hydrogen-rich saline, 5 mL/kg intraperitoneally once daily from reperfusion until sacrifice
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
The introduction discusses theoretical saturation up to 0.8 mM; the actual preparation concentration was not clearly stated in the extracted methods.
Ischemia-reperfusion animals receiving non-hydrogen saline, with sham-operated controls
Outcomes and reported result
Retinal morphology, cell loss, TUNEL staining, 8-OHdG, PARP-1 and caspase-3
The article reports less retinal cell loss, DNA oxidative-stress signal, PARP-1 overactivation and caspase-3 activation with hydrogen-rich saline.
Methods and principal laboratory outcomes checked in the free full text; exact total animal allocation and actual saline H₂ concentration remain unclear.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal injury model with laboratory and histologic outcomes; total animal allocation and actual H₂ concentration require further clarification. It cannot establish benefit in people.
Experimental retinal ischemia-reperfusion injury in rats, not human eye disease.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 25954991 · DOI: 10.3892/mmr.2015.3731
Open-access full text is available through PMC.
Open-access PMC full text and PubMed bibliographic record.
10 August 2026
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