Hydrogen-rich saline promotes recovery of renal function after ischemia-reperfusion injury in rats via anti-apoptosis and anti-inflammation
Li Y et al. · Front Pharmacol. 2016;7:106.
Study at a glance
Preclinical
Rats after right nephrectomy and 45-minute left renal-pedicle occlusion
0.6 mmol/L hydrogen-rich saline, 1 mL/kg intraperitoneally immediately after reperfusion and every 4 hours · 0.6 mmol/L dissolved H₂; saline was saturated for 6 hours at 0.4 MPa and verified by gas chromatography.
Short 24-hour treatment or continuous treatment through 108 hours
The article reports less histologic injury and faster BUN and creatinine recovery with H₂ saline. Renal-function measures worsened again after the short treatment was stopped compared with continuous treatment.
Preclinical renal-ischemia model with nephrectomy, frequent injections and multiple time points. The article contains internally confusing wording about Bcl-2/Bax direction that requires independent verification.
What kind of evidence is this?
Preclinical
Controlled rat unilateral-renal-ischemia experiment comparing short and continuous H₂-saline schedules
Kidney and urinary health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Rats after right nephrectomy and 45-minute left renal-pedicle occlusion
120 rats: sham n=30, ischemia/reperfusion n=30, continuous H₂-saline n=30 and 24-hour H₂-saline n=30
Short 24-hour treatment or continuous treatment through 108 hours
0.6 mmol/L hydrogen-rich saline, 1 mL/kg intraperitoneally immediately after reperfusion and every 4 hours
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
0.6 mmol/L dissolved H₂; saline was saturated for 6 hours at 0.4 MPa and verified by gas chromatography.
Ischemia-reperfusion rats receiving ordinary saline and sham-operated controls
Outcomes and reported result
BUN, creatinine, histology, apoptosis and Bcl-2, Bax, caspases, IL-6 and TNF-α
The article reports less histologic injury and faster BUN and creatinine recovery with H₂ saline. Renal-function measures worsened again after the short treatment was stopped compared with continuous treatment.
The complete open-access article was checked for preparation, concentration, allocation, dose, schedules and principal positive and null results.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical renal-ischemia model with nephrectomy, frequent injections and multiple time points. The article contains internally confusing wording about Bcl-2/Bax direction that requires independent verification.
Renal ischemia-reperfusion after contralateral nephrectomy in rats.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 27148060 · DOI: 10.3389/fphar.2016.00106
Open-access full text is available through PMC and Frontiers.
Open-access PMC/Frontiers full text and PubMed record.
10 August 2026
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