Hydrogen-rich saline promotes recovery of renal function after ischemia-reperfusion injury in rats via anti-apoptosis and anti-inflammation
Li Y et al. · Front Pharmacol. 2016;7:106.
What kind of evidence is this?
Preclinical
Controlled rat unilateral-renal-ischemia experiment comparing short and continuous H₂-saline schedules
Kidney and urinary health
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
Rats after right nephrectomy and 45-minute left renal-pedicle occlusion
120 rats: sham n=30, ischemia/reperfusion n=30, continuous H₂-saline n=30 and 24-hour H₂-saline n=30
Short 24-hour treatment or continuous treatment through 108 hours
0.6 mmol/L hydrogen-rich saline, 1 mL/kg intraperitoneally immediately after reperfusion and every 4 hours
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
0.6 mmol/L dissolved H₂; saline was saturated for 6 hours at 0.4 MPa and verified by gas chromatography.
Not applicable — intraperitoneal saline, not gas inhalation.
No O₂ was co-delivered as part of the intervention.
Ischemia-reperfusion rats receiving ordinary saline and sham-operated controls
Outcomes and reported result
BUN, creatinine, histology, apoptosis and Bcl-2, Bax, caspases, IL-6 and TNF-α
The article reports less histologic injury and faster BUN and creatinine recovery with H₂ saline. Renal-function measures worsened again after the short treatment was stopped compared with continuous treatment.
The complete open-access article was checked for preparation, concentration, allocation, dose, schedules and principal positive and null results.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical renal-ischemia model with nephrectomy, frequent injections and multiple time points. The article contains internally confusing wording about Bcl-2/Bax direction that requires independent verification.
Renal ischemia-reperfusion after contralateral nephrectomy in rats.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 27148060 · DOI: 10.3389/fphar.2016.00106
Open-access full text is available through PMC and Frontiers.
Open-access PMC/Frontiers full text and PubMed record.
2026-08-10