Hydrogen-rich saline alleviates kidney fibrosis following acute kidney injury and retains Klotho expression
Chen J et al. · Front Pharmacol. 2017;8:499.
Study at a glance
Preclinical
Male C57 mice with 35-minute bilateral renal-pedicle occlusion
0.6 mmol/L hydrogen-rich saline, 1 mL/kg intraperitoneally every day · 0.6 mmol/L dissolved H₂.
14 or 28 days
The article reports less fibrosis, lower BUN and creatinine, higher retained Klotho expression and higher LC3-II and Beclin-1 measures with H₂ saline.
Small mouse study. The authors note that steady-state autophagy markers do not prove dynamic autophagic flux and that the mechanistic relationships remain descriptive.
What kind of evidence is this?
Preclinical
Controlled mouse bilateral-renal-ischemia experiment with 14- and 28-day treatment endpoints
Kidney and urinary health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male C57 mice with 35-minute bilateral renal-pedicle occlusion
30 mice across sham, ischemia-reperfusion and ischemia-reperfusion plus H₂-saline groups, n=10 per group
14 or 28 days
0.6 mmol/L hydrogen-rich saline, 1 mL/kg intraperitoneally every day
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
0.6 mmol/L dissolved H₂.
Ischemia-reperfusion mice receiving ordinary saline and sham-operated saline controls
Outcomes and reported result
BUN, creatinine, renal histology and fibrosis, α-SMA, collagen I, Klotho expression and autophagy-associated proteins
The article reports less fibrosis, lower BUN and creatinine, higher retained Klotho expression and higher LC3-II and Beclin-1 measures with H₂ saline.
Dose, allocation, methods, principal results, funding and conflicts checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Small mouse study. The authors note that steady-state autophagy markers do not prove dynamic autophagic flux and that the mechanistic relationships remain descriptive.
Renal ischemia-reperfusion and subsequent fibrosis in mice, not clinical acute kidney injury.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 28848432 · DOI: 10.3389/fphar.2017.00499
Open-access full text is available through PMC and Frontiers.
Open-access PMC/Frontiers full text and PubMed record.
10 August 2026
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