Protective effect of hydrogen-saturated saline on acute lung injury induced by oleic acid in rats.
Ying Y, Xu H, Yao M, Qin Z. · Journal of Orthopaedic Surgery and Research. 2017;12:133.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Respiratory health
Other forms
Positive preclinical signal.
Animal physiologic, tissue and biomarker outcomes.
Methods at a glance
Male Sprague-Dawley rats in an oleic-acid acute-lung-injury model.
40 rats; 10 each in control, injury, intravenous hydrogen-saline and intraperitoneal hydrogen-saline groups.
Three hours after injury induction.
Hydrogen-saturated saline, 5 mL/kg intravenously or intraperitoneally five minutes after injury induction.
H2 dissolved in saline; not Brown's gas.
Hydrogen concentration was maintained at approximately 0.6 mmol/L.
Not applicable — injection.
No oxygen was co-delivered.
Uninjured control and injured saline-control groups.
Outcomes and reported result
Arterial oxygenation, lung histology, edema and inflammatory and oxidative biomarkers.
Both hydrogen-saline routes improved several oxygenation, histologic and inflammatory outcomes; intraperitoneal delivery was stronger on selected measures.
The full article, methods, group comparisons and measured endpoints were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Short-duration animal model; results do not establish benefit in human acute lung injury.
The reported oleic-acid rat model.
SYRCLE animal-study tool — preliminary
Some concerns
Small groups and incomplete reporting of concealment and assessor blinding.
Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 28927460 · DOI: 10.1186/s13018-017-0633-9
Free full article in PubMed Central.
PubMed record and PubMed Central full text; extraction checked 9 August 2026.
2026-08-10