Protective effect of hydrogen-saturated saline on acute lung injury induced by oleic acid in rats.
Ying Y, Xu H, Yao M, Qin Z. · Journal of Orthopaedic Surgery and Research. 2017;12:133.
Study at a glance
Preclinical
Male Sprague-Dawley rats in an oleic-acid acute-lung-injury model.
Hydrogen-saturated saline, 5 mL/kg intravenously or intraperitoneally five minutes after injury induction. · Hydrogen concentration was maintained at approximately 0.6 mmol/L.
Three hours after injury induction.
Both hydrogen-saline routes improved several oxygenation, histologic and inflammatory outcomes; intraperitoneal delivery was stronger on selected measures.
Short-duration animal model; results do not establish benefit in human acute lung injury.
What kind of evidence is this?
Preclinical
Randomized controlled rat study
Respiratory health
Other forms
Positive preclinical signal.
Animal physiologic, tissue and biomarker outcomes.
Methods
Male Sprague-Dawley rats in an oleic-acid acute-lung-injury model.
40 rats; 10 each in control, injury, intravenous hydrogen-saline and intraperitoneal hydrogen-saline groups.
Three hours after injury induction.
Hydrogen-saturated saline, 5 mL/kg intravenously or intraperitoneally five minutes after injury induction.
H2 dissolved in saline; not Brown's gas.
Hydrogen concentration was maintained at approximately 0.6 mmol/L.
Uninjured control and injured saline-control groups.
Outcomes and reported result
Arterial oxygenation, lung histology, edema and inflammatory and oxidative biomarkers.
Both hydrogen-saline routes improved several oxygenation, histologic and inflammatory outcomes; intraperitoneal delivery was stronger on selected measures.
The full article, methods, group comparisons and measured endpoints were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Short-duration animal model; results do not establish benefit in human acute lung injury.
The reported oleic-acid rat model.
SYRCLE animal-study tool — preliminary
Some concerns
Small groups and incomplete reporting of concealment and assessor blinding.
Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 28927460 · DOI: 10.1186/s13018-017-0633-9
Free full article in PubMed Central.
PubMed record and PubMed Central full text; extraction checked 9 August 2026.
10 August 2026
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