Protective effects of hydrogen-rich medium on lipopolysaccharide-induced injury in human periodontal ligament cells
Zhou M, Wang ZL. · West China Journal of Stomatology. 2018;36(2):123-127.
Study at a glance
Preclinical
Primary human periodontal ligament cells at passages 4-5 exposed to E. coli lipopolysaccharide in culture; no patients or animals were treated.
H₂-rich DMEM with 10% fetal bovine serum was used with 1 µg/mL LPS. · Medium was exposed to >99.9999% H₂ for four hours at 0.4 MPa until saturated and stored at 4°C. The article does not report a numerical final dissolved-H₂ concentration.
Proliferation assessed through 72 hours, redox markers at 6, 12 and 24 hours, LDH and apoptosis at 24 hours.
H₂-rich medium improved proliferation at 48/72 hours, lowered apoptosis and increased catalase at 6/12 hours. LDH and SOD did not differ significantly, and catalase at 24 hours did not differ. The English abstract says MDA decreased at six hours, while the Chinese results text says it increased; Hydrogenology therefore treats the MDA direction as internally inconsistent rather than choosing one version.
Two-condition in-vitro design, unclear biological-replicate total, no reported final H₂ concentration, no non-LPS groups, H₂ volatility acknowledged by the authors, and an internal English/Chinese disagreement about the six-hour MDA result. Chinese public/university grants were reported; no explicit conflict statement was identified, so absence is not inferred.
What kind of evidence is this?
Preclinical
Two-condition in-vitro human periodontal-ligament-cell experiment
Oral and dental health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Primary human periodontal ligament cells at passages 4-5 exposed to E. coli lipopolysaccharide in culture; no patients or animals were treated.
Normal-medium plus LPS and H₂-rich-medium plus LPS conditions. The article does not report a single biological-replicate total; some assays were run in triplicate.
Proliferation assessed through 72 hours, redox markers at 6, 12 and 24 hours, LDH and apoptosis at 24 hours.
H₂-rich DMEM with 10% fetal bovine serum was used with 1 µg/mL LPS.
H₂ dissolved in cell-culture medium — H₂ only, not Brown's gas and not inhalation.
Medium was exposed to >99.9999% H₂ for four hours at 0.4 MPa until saturated and stored at 4°C. The article does not report a numerical final dissolved-H₂ concentration.
Ordinary DMEM plus 10% fetal bovine serum and the same 1 µg/mL LPS exposure.
Outcomes and reported result
Cell proliferation, LDH release, apoptosis, catalase, SOD and MDA in supernatant.
H₂-rich medium improved proliferation at 48/72 hours, lowered apoptosis and increased catalase at 6/12 hours. LDH and SOD did not differ significantly, and catalase at 24 hours did not differ. The English abstract says MDA decreased at six hours, while the Chinese results text says it increased; Hydrogenology therefore treats the MDA direction as internally inconsistent rather than choosing one version.
The complete bilingual PMC article was checked for cell conditions, preparation pressure/purity, timing, positive and null outcomes, the English/Chinese MDA inconsistency, funding and available disclosures.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Two-condition in-vitro design, unclear biological-replicate total, no reported final H₂ concentration, no non-LPS groups, H₂ volatility acknowledged by the authors, and an internal English/Chinese disagreement about the six-hour MDA result. Chinese public/university grants were reported; no explicit conflict statement was identified, so absence is not inferred.
LPS-exposed cultured periodontal ligament cells; it does not establish treatment of periodontitis in people.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 29779270 · DOI: 10.7518/hxkq.2018.02.002
Free full bilingual article in PubMed Central.
Complete Chinese/English PMC article and PubMed metadata; full-text extraction checked 9 August 2026.
10 August 2026
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