Hydrogen-rich saline ameliorates hippocampal neuron apoptosis through up-regulating cystathionine β-synthase after cerebral ischemia-reperfusion in rats
Cong HM et al. · Iran J Basic Med Sci. 2020;23(4):494–499.
Study at a glance
Preclinical
Adult male Sprague-Dawley rats after two hours of MCAO and reperfusion
Hydrogen-rich saline 1 mL/kg after the beginning of reperfusion · At least 0.6 mmol/L dissolved H₂; 1 mL/kg. Administration route was not stated in the article’s experiment-design paragraph.
Two hours of occlusion followed by reperfusion; principal assessment at 24 hours
The article reports higher H₂S, CBS, Nrf2, HO-1 and SOD and lower injury, apoptosis, S100β, NSE, ROS and MDA with H₂-rich saline.
Small acute preclinical stroke model with many molecular outcomes and an unreported administration route.
What kind of evidence is this?
Preclinical
Randomized three-group rat middle-cerebral-artery-occlusion experiment
Other neurological conditions
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Adult male Sprague-Dawley rats after two hours of MCAO and reperfusion
36 rats, n=12/group
Two hours of occlusion followed by reperfusion; principal assessment at 24 hours
Hydrogen-rich saline 1 mL/kg after the beginning of reperfusion
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
At least 0.6 mmol/L dissolved H₂; 1 mL/kg. Administration route was not stated in the article’s experiment-design paragraph.
Untreated ischemia-reperfusion and sham surgery
Outcomes and reported result
H₂S, S100β, NSE, ROS, MDA, SOD, hippocampal histology and apoptosis, CBS, Nrf2 and HO-1
The article reports higher H₂S, CBS, Nrf2, HO-1 and SOD and lower injury, apoptosis, S100β, NSE, ROS and MDA with H₂-rich saline.
Allocation, concentration threshold, dose, timing, methods and principal outcomes checked in the open-access full text; route is not reported.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Small acute preclinical stroke model with many molecular outcomes and an unreported administration route.
Cerebral ischemia-reperfusion in rats, not stroke care in people.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 32489564 · DOI: 10.22038/ijbms.2020.41751.9857
Open-access full text is available through PMC.
Open-access PMC article and PubMed record; no retraction or expression of concern was shown in the checked records on 7 August 2026.
10 August 2026
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