Hydrogen-rich saline ameliorates hippocampal neuron apoptosis through up-regulating cystathionine β-synthase after cerebral ischemia-reperfusion in rats
Cong HM et al. · Iran J Basic Med Sci. 2020;23(4):494–499.
What kind of evidence is this?
Preclinical
Randomized three-group rat middle-cerebral-artery-occlusion experiment
Other neurological conditions
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
Adult male Sprague-Dawley rats after two hours of MCAO and reperfusion
36 rats, n=12/group
Two hours of occlusion followed by reperfusion; principal assessment at 24 hours
Hydrogen-rich saline 1 mL/kg after the beginning of reperfusion
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
At least 0.6 mmol/L dissolved H₂; 1 mL/kg. Administration route was not stated in the article’s experiment-design paragraph.
Not applicable — saline administration, not gas inhalation.
No O₂ was co-delivered as part of the H₂ intervention.
Untreated ischemia-reperfusion and sham surgery
Outcomes and reported result
H₂S, S100β, NSE, ROS, MDA, SOD, hippocampal histology and apoptosis, CBS, Nrf2 and HO-1
The article reports higher H₂S, CBS, Nrf2, HO-1 and SOD and lower injury, apoptosis, S100β, NSE, ROS and MDA with H₂-rich saline.
Allocation, concentration threshold, dose, timing, methods and principal outcomes checked in the open-access full text; route is not reported.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Small acute preclinical stroke model with many molecular outcomes and an unreported administration route.
Cerebral ischemia-reperfusion in rats, not stroke care in people.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 32489564 · DOI: 10.22038/ijbms.2020.41751.9857
Open-access full text is available through PMC.
Open-access PMC article and PubMed record; no retraction or expression of concern was shown in the checked records on 7 August 2026.
2026-08-10