Hydrogenology
Hydrogenology editorial study record

Nanoparticulate MgH2 ameliorates anxiety/depression-like behaviors in a mouse model of multiple sclerosis by regulating microglial polarization and oxidative stress.

Li Z, Chen K, Shao Q, Lu H, Zhang X, Pu Y, Sun X, He H, Cao L. · Journal of Neuroinflammation. 2023;20:26.

PreclinicalOther formsPublished 2023
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Controlled mouse and cell study

Research topic

Other neurological conditions

Administration classification

Other forms

Study result signal

Mixed preclinical signal.

Outcome type

Animal functional, disease-score, tissue and biomarker outcomes.

Reported in the source

Methods at a glance

Population or model

Mouse experimental autoimmune encephalomyelitis, restraint and lysolecithin models plus cell experiments.

Sample

Multiple experiments; the EAE comparison included 12 supplemented and 11 control-model mice at analysis.

Duration

Up to 30 days in the EAE experiment, with model-specific schedules elsewhere.

Intervention

AIN93G feed containing 0.5% magnesium hydride nanoparticles.

Hydrogen form

Hydrogen generated by magnesium hydride; not Brown's gas.

H₂ specification

Magnesium and hydrogen effects were not fully separable.

H₂ flow

Not applicable — dietary agent.

O₂ delivered with H₂

No oxygen was co-delivered.

Comparator

Standard feed and model-specific control conditions.

Reported, not endorsed

Outcomes and reported result

Outcomes measured

EAE incidence and score, anxiety/depression-like behavior, inflammation, oxidative stress and remyelination.

Reported result

EAE and selected behavioral and inflammatory outcomes improved, but oligodendrocyte differentiation and remyelination outcomes were not improved in the tested models.

Results-extraction completeness

The full article, multiple models, methods and positive and null findings were checked.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Preclinical, multi-model evidence; magnesium-hydride and hydrogen effects cannot be separated.

Applies directly to

The reported mouse and cell neuroinflammation models.

Preliminary appraisal framework

SYRCLE animal-study tool — preliminary

Preliminary risk-of-bias status

Some concerns

Appraisal rationale

Model-specific attrition and unclear allocation concealment and assessor blinding.

Appraisal domains

Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 36710351 · DOI: 10.1186/s12974-023-02696-y

Publisher access

Free full article in PubMed Central.

Extraction basis

PubMed record and PubMed Central full text; extraction checked 9 August 2026.

Record revision

2026-08-10