Hydrogen gas reduced acute-hyperglycemia-enhanced hemorrhagic transformation in a focal-ischemia rat model
Chen CH et al. · Neuroscience. 2010;169(1):402–414.
Study at a glance
Preclinical
72 Sprague-Dawley rats receiving dextrose and 90-minute focal cerebral ischemia
Premixed gas inhalation for 1 or 2 hours immediately after ischemia · 2.9% H₂ + 21% O₂ + 76.1% N₂.
One or two hours of inhalation during reperfusion; outcomes at 24 hours
Two-hour H₂ inhalation reduced hemorrhage and infarct volumes and improved neurological scores; one hour reduced hemorrhage but not infarct volume. MMP-9 and several oxidative markers decreased, while MMP-2, ZO-1, occludin, collagen IV and AQP4 did not significantly change.
Acute rat stroke model with experimentally induced hyperglycemia and multiple laboratory outcomes; no human participants.
What kind of evidence is this?
Preclinical
Controlled rat hyperglycemia plus transient middle-cerebral-artery-occlusion experiment
Other neurological conditions
Inhaled H₂
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
72 Sprague-Dawley rats receiving dextrose and 90-minute focal cerebral ischemia
72 rats across sham, sham plus H₂, ischemia and ischemia plus H₂ groups
One or two hours of inhalation during reperfusion; outcomes at 24 hours
Premixed gas inhalation for 1 or 2 hours immediately after ischemia
H₂ diluted with O₂ and N₂ — not Brown’s gas.
2.9% H₂ + 21% O₂ + 76.1% N₂.
Not reported — premixed gas composition is given, but H₂ flow in mL/min is not.
21% O₂ was co-delivered; O₂ flow in mL/min was not reported.
Corresponding premixed gas without H₂ and sham groups
Outcomes and reported result
Infarct and hemorrhage volumes, neurological score, glucose, oxidative markers, MMP-2/MMP-9 and blood-brain-barrier proteins
Two-hour H₂ inhalation reduced hemorrhage and infarct volumes and improved neurological scores; one hour reduced hemorrhage but not infarct volume. MMP-9 and several oxidative markers decreased, while MMP-2, ZO-1, occludin, collagen IV and AQP4 did not significantly change.
Gas composition, allocation, methods, positive and null outcomes, funding and figures checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Acute rat stroke model with experimentally induced hyperglycemia and multiple laboratory outcomes; no human participants.
Transient focal cerebral ischemia with acute hyperglycemia in rats.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 20423721 · DOI: 10.1016/j.neuroscience.2010.04.043
Free full text is available through PMC.
Open-access PMC article and PubMed record.
10 August 2026
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