Protective effects of hydrogen-rich saline in uncontrolled hemorrhagic shock
Du Z et al. · Exp Ther Med. 2014;7(5):1253–1258.
Study at a glance
Preclinical
Male Wistar rats with arterial bleeding and tail amputation
Hydrogen-rich saline given intravenously or intraperitoneally during resuscitation · Above 0.6 mmol/L dissolved H₂; administered volume requires further extraction.
Hemodynamic and biomarker measurements through 210 minutes; survival checked at 24 hours
Inflammatory and oxidative-stress markers favored H₂ saline, with some measures favoring intravenous delivery. Survival was 100% in every group and hemodynamics did not significantly differ among experimental groups.
Mild rat shock model with no survival or hemodynamic difference, short follow-up and biomarker-focused findings. The route comparison should not be generalized clinically.
What kind of evidence is this?
Preclinical
Randomized rat hemorrhagic-shock experiment comparing intravenous and intraperitoneal H₂ saline
Critical care and ischemia-reperfusion
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male Wistar rats with arterial bleeding and tail amputation
50 rats in five groups, n=10 per group
Hemodynamic and biomarker measurements through 210 minutes; survival checked at 24 hours
Hydrogen-rich saline given intravenously or intraperitoneally during resuscitation
H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.
Above 0.6 mmol/L dissolved H₂; administered volume requires further extraction.
Sham group and shock groups receiving ordinary saline by the corresponding route
Outcomes and reported result
Survival, arterial pressure, heart rate, IL-6, TNF-α, SOD and MDA
Inflammatory and oxidative-stress markers favored H₂ saline, with some measures favoring intravenous delivery. Survival was 100% in every group and hemodynamics did not significantly differ among experimental groups.
Concentration, allocation, full methods and positive and null results checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Mild rat shock model with no survival or hemodynamic difference, short follow-up and biomarker-focused findings. The route comparison should not be generalized clinically.
Experimental uncontrolled hemorrhagic shock in rats.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 24940421 · DOI: 10.3892/etm.2014.1572
Open-access full text is available through PMC.
Open-access PMC full text and PubMed record.
10 August 2026
Help people check the facts before they buy.
Hydrogenology keeps 665 source-checked records accessible without advertising or product promotion.
Help us correct the recordReport a discrepancy
Send the exact difference between this page and the linked source. The study reference is attached automatically.