Hydrogenology
Hydrogenology editorial study record

Protective effects of hydrogen-rich saline in uncontrolled hemorrhagic shock

Du Z et al. · Exp Ther Med. 2014;7(5):1253–1258.

PreclinicalOther formsPublished 2014
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Randomized rat hemorrhagic-shock experiment comparing intravenous and intraperitoneal H₂ saline

Research topic

Critical care and ischemia-reperfusion

Administration classification

Other forms

Study result signal

Not reported in this record.

Outcome type

Not reported in this record.

Reported in the source

Methods at a glance

Population or model

Male Wistar rats with arterial bleeding and tail amputation

Sample

50 rats in five groups, n=10 per group

Duration

Hemodynamic and biomarker measurements through 210 minutes; survival checked at 24 hours

Intervention

Hydrogen-rich saline given intravenously or intraperitoneally during resuscitation

Hydrogen form

H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.

H₂ specification

Above 0.6 mmol/L dissolved H₂; administered volume requires further extraction.

H₂ flow

Not applicable — saline administration, not gas inhalation.

O₂ delivered with H₂

No O₂ was co-delivered as part of the intervention.

Comparator

Sham group and shock groups receiving ordinary saline by the corresponding route

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Survival, arterial pressure, heart rate, IL-6, TNF-α, SOD and MDA

Reported result

Inflammatory and oxidative-stress markers favored H₂ saline, with some measures favoring intravenous delivery. Survival was 100% in every group and hemodynamics did not significantly differ among experimental groups.

Results-extraction completeness

Concentration, allocation, full methods and positive and null results checked in the open-access full text.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Mild rat shock model with no survival or hemodynamic difference, short follow-up and biomarker-focused findings. The route comparison should not be generalized clinically.

Applies directly to

Experimental uncontrolled hemorrhagic shock in rats.

Preliminary appraisal framework

Not reported in this record.

Preliminary risk-of-bias status

Not reported in this record.

Appraisal rationale

Not reported in this record.

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 24940421 · DOI: 10.3892/etm.2014.1572

Publisher access

Open-access full text is available through PMC.

Extraction basis

Open-access PMC full text and PubMed record.

Record revision

2026-08-10

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