Evaluation of the effect of enriched hydrogen saline solution on distant organ (lung) damage in skeletal muscle ischemia reperfusion in rats
Özer A, Erel S, Küçük A, Demirtaş H, Sezen ŞC, Boyunağa H, Oktar GL, Arslan M. · 2024.
Study at a glance
Preclinical
Rats with lower-limb ischemia-reperfusion and subsequent lung assessment.
Hydrogen-enriched saline around the ischemia-reperfusion procedure. · 0.6 mmol/L; 10 mL administered under the article protocol.
30 minutes pretreatment, 120 minutes ischemia and 120 minutes reperfusion.
Several lung-injury, oxidative and inflammatory measures favored hydrogen-enriched saline over untreated ischemia-reperfusion.
Animal evidence only; short experimental follow-up and several biochemical endpoints.
What kind of evidence is this?
Preclinical
Controlled rat ischemia-reperfusion study
Critical care and ischemia-reperfusion
Other forms
Positive preclinical signal.
Remote-organ tissue and biomarker outcomes in rats.
Methods
Rats with lower-limb ischemia-reperfusion and subsequent lung assessment.
Study groups and assay counts were checked in the full article.
30 minutes pretreatment, 120 minutes ischemia and 120 minutes reperfusion.
Hydrogen-enriched saline around the ischemia-reperfusion procedure.
H₂ dissolved in saline.
0.6 mmol/L; 10 mL administered under the article protocol.
Sham and untreated ischemia-reperfusion groups.
Outcomes and reported result
Lung histology, oxidative-stress markers and inflammatory measures.
Several lung-injury, oxidative and inflammatory measures favored hydrogen-enriched saline over untreated ischemia-reperfusion.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal evidence only; short experimental follow-up and several biochemical endpoints.
The reported rat limb-ischemia model only.
SYRCLE animal-study tool — preliminary
Some concerns
Comparator groups were reported, but concealment and blinded histological assessment were not established.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 38807538 · DOI: 10.1177/00368504241257060
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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