Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice
Zhang JY et al. · World J Gastroenterol. 2015;21(14):4195–4209.
Study at a glance
Preclinical
Male C57BL/6 mice given intraperitoneal acetaminophen
Hydrogen-rich water 5 mL/kg injected intraperitoneally twice daily for three days after acetaminophen · 0.83–0.91 mmol/L dissolved H₂; 5 mL/kg at 08:00 and 17:00.
Twice daily for three days; survival followed for five days and tissue outcomes at 24, 48 and 72 hours
The article reports 60% versus 26.67% five-day survival after lethal acetaminophen and lower injury, oxidative and inflammatory measures with H₂-rich water, alongside proliferation markers.
Despite the title, the H₂-rich water was injected intraperitoneally, not drunk. This is a mouse toxicology model with multiple outcomes.
What kind of evidence is this?
Preclinical
Randomized mouse lethal- and sublethal-acetaminophen experiments
Liver health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male C57BL/6 mice given intraperitoneal acetaminophen
Survival experiment: control groups n=5 and acetaminophen groups n=15; sublethal outcome groups commonly n=6
Twice daily for three days; survival followed for five days and tissue outcomes at 24, 48 and 72 hours
Hydrogen-rich water 5 mL/kg injected intraperitoneally twice daily for three days after acetaminophen
H₂ dissolved in water and injected intraperitoneally — H₂ only, not Brown’s gas, not inhalation and not oral drinking.
0.83–0.91 mmol/L dissolved H₂; 5 mL/kg at 08:00 and 17:00.
Equal-volume normal saline, with and without acetaminophen
Outcomes and reported result
Survival, liver enzymes and histology, oxidative and inflammatory markers, organelles, JNK, connexin-32, CYP2E1 and hepatocyte proliferation
The article reports 60% versus 26.67% five-day survival after lethal acetaminophen and lower injury, oxidative and inflammatory measures with H₂-rich water, alongside proliferation markers.
Concentration, dose, injection route, allocation, schedule and principal numerical outcomes checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Despite the title, the H₂-rich water was injected intraperitoneally, not drunk. This is a mouse toxicology model with multiple outcomes.
Acetaminophen hepatotoxicity in mice, not overdose treatment in people.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 25892869 · DOI: 10.3748/wjg.v21.i14.4195
Open-access full text is available through PMC and the publisher.
Open-access PMC article and PubMed record; no retraction or expression of concern was shown in the checked records on 7 August 2026.
10 August 2026
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