Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice
Peng Z, Chen W, Wang L, Ye Z, Gao S, Sun X, Guo Z. · International Journal of Clinical and Experimental Pathology. 2015;8(3):2680-2689.
Study at a glance
Preclinical
Male C57BL/6 mice in a five-day glyoxylate-induced renal calcium-oxalate deposition model.
A 67% H₂/33% O₂ mixture was inhaled in a closed box for 30 minutes daily for seven days, starting two days before the five-day glyoxylate course. · The total mixture entered the chamber at 200 mL/min. Directly from the reported 67%/33% composition, H₂ flow was 134 mL/min and O₂ flow was 66 mL/min. The control mixture was 67% N₂/33% O₂ at the same total flow.
Thirty minutes daily for seven consecutive days; glyoxylate 100 mg/kg/day was given intraperitoneally on days 3-7.
Brown's-gas pretreatment reduced renal crystal deposition, calcium, KIM-1, apoptosis and several oxidative/inflammatory markers and increased antioxidant measures and IL-10. H₂/O₂ alone did not change urinary calcium/phosphate ratios, renal calcium or KIM-1; renal MPO did not differ significantly.
Small n=6 groups, prophylactic treatment beginning before injury, short chemical-crystallization model, many surrogate endpoints and no comparison with established stone-prevention treatments. Chinese national, Shanghai and hospital grants were reported; authors disclosed no conflicts.
What kind of evidence is this?
Preclinical
Randomized four-group controlled mouse pretreatment experiment
Kidney and urinary health
Inhaled H₂
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male C57BL/6 mice in a five-day glyoxylate-induced renal calcium-oxalate deposition model.
24 mice randomized to four groups of n=6: control gas, Brown's gas, glyoxylate plus control gas, or glyoxylate plus Brown's gas.
Thirty minutes daily for seven consecutive days; glyoxylate 100 mg/kg/day was given intraperitoneally on days 3-7.
A 67% H₂/33% O₂ mixture was inhaled in a closed box for 30 minutes daily for seven days, starting two days before the five-day glyoxylate course.
Brown's gas / oxyhydrogen generated from water: approximately 66.7% H₂ and 33.3% O₂ in the source's 2:1 H₂:O₂ proportions.
The total mixture entered the chamber at 200 mL/min. Directly from the reported 67%/33% composition, H₂ flow was 134 mL/min and O₂ flow was 66 mL/min. The control mixture was 67% N₂/33% O₂ at the same total flow.
134 mL/min.
66 mL/min.
Matching 67% N₂/33% O₂ control gas with and without glyoxylate, plus Brown's gas without glyoxylate.
Outcomes and reported result
Urinary minerals, renal calcium deposition, serum KIM-1, TUNEL apoptosis, MDA, 8-OHdG, SOD, GSH, catalase, MPO, OPN, CD44, MCP-1 and IL-10.
Brown's-gas pretreatment reduced renal crystal deposition, calcium, KIM-1, apoptosis and several oxidative/inflammatory markers and increased antioxidant measures and IL-10. H₂/O₂ alone did not change urinary calcium/phosphate ratios, renal calcium or KIM-1; renal MPO did not differ significantly.
The complete free PMC article was checked for randomization, exact gas generator/composition, total flow, directly calculated H₂/O₂ flows, dosing schedule, positive and null outcomes, funding and conflict declaration.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Small n=6 groups, prophylactic treatment beginning before injury, short chemical-crystallization model, many surrogate endpoints and no comparison with established stone-prevention treatments. Chinese national, Shanghai and hospital grants were reported; authors disclosed no conflicts.
Glyoxylate-induced calcium-oxalate deposition in male mice; it does not establish kidney-stone prevention or treatment in humans.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 26045773
Free full article in PubMed Central.
Complete PMC article and PubMed metadata; full-text extraction checked 9 August 2026.
10 August 2026
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