Intravenous infusion of H_2-saline suppresses oxidative stress and elevates antioxidant potential in Thoroughbred horses after racing exercise
Yamazaki M, Kusano K, Ishibashi T, Kiuchi M, Koyama K. · 2015.
Study at a glance
Preclinical
Thoroughbred racehorses after racing exercise.
Intravenous infusion of hydrogen-rich saline after racing. · Approximately 0.6 ppm in the administered saline.
Acute post-racing infusion with short laboratory follow-up.
Some antioxidant measures changed, while the article reports no significant difference for at least one emphasized between-condition comparison.
Very small animal crossover study with surrogate laboratory outcomes only.
What kind of evidence is this?
Preclinical
Randomized blinded crossover horse study
Exercise and recovery
Other forms
Mixed or mostly neutral preclinical biomarker signal.
Animal exercise biomarker outcomes.
Methods
Thoroughbred racehorses after racing exercise.
Small crossover cohorts; group-specific counts were checked in the full article.
Acute post-racing infusion with short laboratory follow-up.
Intravenous infusion of hydrogen-rich saline after racing.
H₂ dissolved in saline.
Approximately 0.6 ppm in the administered saline.
Placebo saline in a crossover design.
Outcomes and reported result
Oxidative-stress and antioxidant blood measures.
Some antioxidant measures changed, while the article reports no significant difference for at least one emphasized between-condition comparison.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Very small animal crossover study with surrogate laboratory outcomes only.
Thoroughbred horses under this post-race infusion protocol.
SYRCLE animal-study tool — preliminary
Some concerns
Randomization and blinding were reported, but the small sample and multiple biomarkers create imprecision and selective-reporting concerns.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 26493164 · DOI: 10.1038/srep15514
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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