Hydrogen-rich medium alleviates high glucose-induced oxidative stress and parthanatos in rat Schwann cells in vitro
Li Q, Jiao Y, Yu Y, Wang G, Yu Y. · Molecular Medicine Reports. 2019;19(1):338–344.
Study at a glance
Preclinical
Primary rat Schwann cells exposed for 48 hours to normal-glucose or high-glucose culture conditions as an in-vitro model relevant to diabetic peripheral neuropathy.
High-glucose cells were cultured for 48 hours in medium saturated with molecular H₂ at 0.6 mM. · For preparation, H₂ at 1 L/min was mixed with air at 1 L/min and dissolved in medium for four hours at 0.4 MPa; the stored medium was maintained at 0.6 mM H₂.
48-hour culture treatment; H₂-rich medium was freshly prepared weekly.
Under high glucose, H₂-rich medium improved viability and reduced cytotoxicity, oxidative markers, 8-OHdG, PAR and AIF nuclear translocation. The experiment did not test neuropathy, nerve function or clinical outcomes in animals or people.
Small in-vitro replicate counts, one cell type, high-glucose model, multiple mechanistic endpoints and no in-vivo dose, exposure, safety or functional outcome. Chinese public and university grants funded the work; authors declared no competing interests and identified the commercial H₂-generator manufacturer.
What kind of evidence is this?
Preclinical
Randomized four-condition primary rat Schwann-cell experiment
Metabolic and lipid health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Primary rat Schwann cells exposed for 48 hours to normal-glucose or high-glucose culture conditions as an in-vitro model relevant to diabetic peripheral neuropathy.
Outcome-specific laboratory replicates were n=5 per condition for viability/cytotoxicity and generally n=3 per condition for oxidative, DNA-damage and protein measures; no animal treatment experiment was performed.
48-hour culture treatment; H₂-rich medium was freshly prepared weekly.
High-glucose cells were cultured for 48 hours in medium saturated with molecular H₂ at 0.6 mM.
H₂ dissolved in cell-culture medium — H₂ only, not Brown's gas and not inhalation.
For preparation, H₂ at 1 L/min was mixed with air at 1 L/min and dissolved in medium for four hours at 0.4 MPa; the stored medium was maintained at 0.6 mM H₂.
Normal-glucose medium, H₂-rich normal-glucose medium and high-glucose medium without H₂.
Outcomes and reported result
Cell viability, LDH cytotoxicity, hydroxyl-radical and peroxynitrite measures, 8-OHdG, PAR expression and AIF nuclear translocation.
Under high glucose, H₂-rich medium improved viability and reduced cytotoxicity, oxidative markers, 8-OHdG, PAR and AIF nuclear translocation. The experiment did not test neuropathy, nerve function or clinical outcomes in animals or people.
The complete free PMC article and figures were checked for culture allocation, preparation flows and concentration, outcome-specific replicate counts, results, funding and conflicts.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Small in-vitro replicate counts, one cell type, high-glucose model, multiple mechanistic endpoints and no in-vivo dose, exposure, safety or functional outcome. Chinese public and university grants funded the work; authors declared no competing interests and identified the commercial H₂-generator manufacturer.
Primary rat Schwann cells in culture; it does not establish prevention or treatment of diabetic peripheral neuropathy in people.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 30431142 · DOI: 10.3892/mmr.2018.9631
Free full article in PubMed Central.
Complete PMC article and PubMed metadata; full-text extraction checked 9 August 2026.
10 August 2026
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