Molecular hydrogen suppresses free-radical-induced cell death by mitigating fatty acid peroxidation and mitochondrial dysfunction
Iuchi K, Nishimaki K, Kamimura N, Ohta S. · Can J Physiol Pharmacol. 2019;97(10):999–1005.
Study at a glance
Preclinical
THP-1 cells, with confirmatory experiments in human aortic endothelial cells.
Cell exposure to 75% or 10% H₂-containing gas. · 75% or 10% H₂, depending on the experiment.
4.5 to 18 hours depending on assay.
H₂ exposure reduced several free-radical-related cell-injury measures in the tested systems.
Cell-only evidence with high gas concentrations; it does not establish a clinical effect.
What kind of evidence is this?
Preclinical
Controlled cell-culture study
Cardiovascular and circulatory health
Other forms
Positive cell-culture signal.
Cell viability and mechanistic laboratory outcomes.
Methods
THP-1 cells, with confirmatory experiments in human aortic endothelial cells.
Three to four independent replicate experiments per reported assay.
4.5 to 18 hours depending on assay.
Cell exposure to 75% or 10% H₂-containing gas.
H₂ gas applied to cell-culture systems.
75% or 10% H₂, depending on the experiment.
0% H₂ gas and oxidative-injury controls.
Outcomes and reported result
Cell death, LDH release, lipid peroxidation and mitochondrial function.
H₂ exposure reduced several free-radical-related cell-injury measures in the tested systems.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Cell-only evidence with high gas concentrations; it does not establish a clinical effect.
The tested cell systems and exposure conditions only.
In-vitro study appraisal — preliminary
Some concerns
Small replicate counts, assay-level multiplicity and generally unreported blinding.
Independent replicate reporting checked · Exposure and comparator checked · Assay blinding generally unreported · Multiplicity and selective reporting remain possible · External validity is limited to the tested cells
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 31295412 · DOI: 10.1139/cjpp-2018-0741
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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