Hydrogen-rich saline alleviates inflammation and apoptosis in myocardial ischemia-reperfusion injury via PINK-mediated autophagy
Yao L et al. · Int J Mol Med. 2019;44(3):1048–1062.
Study at a glance
Preclinical
Rats with 30 minutes of myocardial ischemia and 24 hours of reperfusion; cultured H9C2 cardiomyoblasts
0.6 mmol/L hydrogen-rich saline, 10 mL/kg intraperitoneally 5 minutes before reperfusion; cells received 0.6 mmol/L H₂-rich medium · 0.6 mmol/L dissolved H₂.
Single pre-reperfusion injection; principal in-vivo assessment after 24 hours of reperfusion
The article reports smaller infarcts and favorable cardiac-function, inflammatory, apoptotic and cell-viability measures with H₂. PINK1 pathway manipulation attenuated several reported effects.
Preclinical study combining a small rat cohort and cultured cells, with multiple mechanistic outcomes and no human participants.
What kind of evidence is this?
Preclinical
Controlled rat myocardial ischemia-reperfusion experiment plus H9C2 hypoxia-reoxygenation cell experiments
Cardiovascular and circulatory health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Rats with 30 minutes of myocardial ischemia and 24 hours of reperfusion; cultured H9C2 cardiomyoblasts
24 rats randomized to four groups, n=6 per group; cell experiments used separate repeated comparisons
Single pre-reperfusion injection; principal in-vivo assessment after 24 hours of reperfusion
0.6 mmol/L hydrogen-rich saline, 10 mL/kg intraperitoneally 5 minutes before reperfusion; cells received 0.6 mmol/L H₂-rich medium
H₂ dissolved in saline or culture medium — H₂ only, not Brown’s gas and not inhalation.
0.6 mmol/L dissolved H₂.
Control and ischemia-reperfusion groups receiving ordinary saline; corresponding cell-culture controls
Outcomes and reported result
Infarct size, cardiac function, cTnI, CK-MB, cytokines, apoptosis, cell viability and PINK1/Parkin autophagy markers
The article reports smaller infarcts and favorable cardiac-function, inflammatory, apoptotic and cell-viability measures with H₂. PINK1 pathway manipulation attenuated several reported effects.
Dose, allocation, methods, figures and principal results checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical study combining a small rat cohort and cultured cells, with multiple mechanistic outcomes and no human participants.
Experimental myocardial ischemia-reperfusion in rats and hypoxia-reoxygenation in cultured cells.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 31524220 · DOI: 10.3892/ijmm.2019.4264
Open-access full text is available through PMC.
Open-access PMC full text and PubMed record.
10 August 2026
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