Protective effects of molecular hydrogen on hepatotoxicity induced by sub-chronic exposure to chlorpyrifos in rats
Xun ZM, Xie F, Zhao PX, Liu MY, Li ZY, Song JM, Kong XM, Ma XM, Li XY. · Ann Agric Environ Med. 2020;27(3):368–373.
Study at a glance
Preclinical
Male Wistar rats exposed to chlorpyrifos.
Hydrogen-rich water available for one hour twice daily during chlorpyrifos exposure. · 0.55–0.65 mmol/L, pH about 8, prepared with a magnesium stick.
Eight weeks.
Several liver-injury and oxidative measures favored hydrogen-rich water, while not every measured endpoint differed.
Animal toxicology evidence only; water alkalinity and magnesium-based preparation may co-vary with H₂.
What kind of evidence is this?
Preclinical
Controlled rat study
Liver health
H₂-rich water
Mixed positive preclinical signal.
Tissue and biochemical outcomes in a toxicology model.
Methods
Male Wistar rats exposed to chlorpyrifos.
30 rats; three groups of 10.
Eight weeks.
Hydrogen-rich water available for one hour twice daily during chlorpyrifos exposure.
H₂ dissolved in drinking water.
0.55–0.65 mmol/L, pH about 8, prepared with a magnesium stick.
Untreated control and chlorpyrifos-only groups.
Outcomes and reported result
Liver histology, enzymes and oxidative and antioxidant markers.
Several liver-injury and oxidative measures favored hydrogen-rich water, while not every measured endpoint differed.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal toxicology evidence only; water alkalinity and magnesium-based preparation may co-vary with H₂.
The reported chlorpyrifos rat model only.
SYRCLE animal-study tool — preliminary
Some concerns
Group controls were present, but randomization, concealment and assessor blinding were not fully described.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 32955216 · DOI: 10.26444/aaem/125504
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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