Hydrogen Attenuates Endotoxin-Induced Lung Injury by Activating Thioredoxin 1 and Decreasing Tissue Factor Expression
Li Q, Hu L, Li J, Yu P, Hu F, Wan B, Xu M, Cheng H, Yu W, Jiang L, Shi Y, Li J, Duan M, Long Y, Liu WT. · 2021.
Study at a glance
Preclinical
Mice with endotoxin-induced acute lung injury and related cell experiments.
4% hydrogen gas inhalation after endotoxin exposure, with related cell experiments. · 4% H₂; the methods report gas delivery at 400 mL/min for the chamber system.
Acute and survival follow-up under article-specific schedules.
The article reports higher survival and favorable lung-injury, inflammatory and signaling measures with hydrogen in the tested model.
Preclinical evidence only; multiple cohorts and mechanistic endpoints.
What kind of evidence is this?
Preclinical
Controlled mouse and cell study
Respiratory health
Inhaled H₂
Positive preclinical signal.
Animal survival, tissue and mechanistic outcomes.
Methods
Mice with endotoxin-induced acute lung injury and related cell experiments.
Multiple animal cohorts and cell assays; totals vary by experiment.
Acute and survival follow-up under article-specific schedules.
4% hydrogen gas inhalation after endotoxin exposure, with related cell experiments.
Inhaled molecular H₂.
4% H₂; the methods report gas delivery at 400 mL/min for the chamber system.
400 mL/min total chamber delivery reported; the hydrogen fraction was 4%.
Carrier-gas composition is reported in the full methods; no Brown's-gas classification is inferred.
Endotoxin injury without H₂ and laboratory controls.
Outcomes and reported result
Survival, lung injury, inflammation, coagulation-related markers and thioredoxin signaling.
The article reports higher survival and favorable lung-injury, inflammatory and signaling measures with hydrogen in the tested model.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; multiple cohorts and mechanistic endpoints.
The reported endotoxin mouse-lung-injury model only.
SYRCLE animal-study tool — preliminary
Some concerns
Controlled groups were used, while concealment, blinding and multiplicity handling were not fully established.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 33767697 · DOI: 10.3389/fimmu.2021.625957
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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