Hydrogen gas alleviates acute alcohol-induced liver injury by inhibiting JNK activation
Zhang Y, Bi M, Chen Z, Dai M, Zhou G, Hu Y, Yang H, Guan W. · Experimental and therapeutic medicine. 2021;21(5):453.
Study at a glance
Preclinical
Twenty-eight mice assigned to control, alcohol, alcohol-plus-H₂ and H₂-only groups (n=7 each).
H₂ gas was administered by intraperitoneal injection at 1.0 mL/100 g body weight once daily for 4 days; on Day 4 it was given 30 minutes before the first ethanol gavage. · Administered H₂ volume: 1.0 mL/100 g body weight. The source gas used to prepare the injection was reported as 99.999% purity; purity is not the administered tissue concentration.
Once daily for 4 days.
Article-reported result excerpt (24 words maximum): “Western blotting data demonstrated that acute ethanol treatment significantly increased hepatic JNK phosphorylation, which was significantly prevented by intraperitoneal injection with H_2 ([Fig.” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
What kind of evidence is this?
Preclinical
Controlled preclinical study
Metabolic and lipid health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Twenty-eight mice assigned to control, alcohol, alcohol-plus-H₂ and H₂-only groups (n=7 each).
28 mice; four groups of 7.
Once daily for 4 days.
H₂ gas was administered by intraperitoneal injection at 1.0 mL/100 g body weight once daily for 4 days; on Day 4 it was given 30 minutes before the first ethanol gavage.
an injected or infused H₂ intervention — H₂ only unless the full text explicitly reports oxygen co-delivery.
Administered H₂ volume: 1.0 mL/100 g body weight. The source gas used to prepare the injection was reported as 99.999% purity; purity is not the administered tissue concentration.
Control, alcohol-only and H₂-only groups alongside the alcohol-plus-H₂ group.
Outcomes and reported result
survival or mortality, clinical symptoms or functional scores, tumor growth or cancer markers, inflammation and cytokines, oxidative-stress and antioxidant markers, apoptosis or cell injury
Article-reported result excerpt (24 words maximum): “Western blotting data demonstrated that acute ethanol treatment significantly increased hepatic JNK phosphorylation, which was significantly prevented by intraperitoneal injection with H_2 ([Fig.” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 33767761 · DOI: 10.3892/etm.2021.9884
A free full article is available through PubMed Central.
Official PubMed/PMC full article; record re-audited and corrected 25 August 2026.
25 August 2026
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