The Role of Hyperthermia in Methamphetamine-Induced Depression-Like Behaviors: Protective Effects of Coral Calcium Hydride.
Wang X, Tong B, Hui R, Hou C, Zhang Z, Zhang L, Xie B, Ni Z, Cong B, Ma C, Wen D. · Frontiers in Molecular Neuroscience. 2021;14:808807.
What kind of evidence is this?
Preclinical
Controlled mouse experiments
Sleep and mood research
Other forms
Mixed positive preclinical signal.
Animal functional behavior and biomarker outcomes.
Methods at a glance
Male mice in methamphetamine hyperthermia and depression-like behavior models.
390 mice distributed across multiple experiments.
Acute and repeated schedules varied by experiment.
Coral calcium hydride, 100 or 200 mg/kg by gavage, before or after methamphetamine depending on experiment.
Hydrogen generated from coral calcium hydride; not Brown's gas.
The product combines coral calcium and hydrogen generation; the article included coral-calcium controls in selected experiments.
Not applicable — gavage.
No oxygen was co-delivered.
Vehicle, methamphetamine and coral-calcium controls as reported by experiment.
Outcomes and reported result
Body temperature, tail-suspension and forced-swim behavior, locomotion and molecular markers.
The higher dose affected hyperthermia and both tested doses affected selected depression-like behavior measures; locomotion was not improved.
The full article, all experimental methods, behavioral outcomes and null locomotor finding were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical behavioral models do not establish an antidepressant effect in people; product components and hydrogen generation are not fully separable.
The reported methamphetamine mouse models.
SYRCLE animal-study tool — preliminary
Some concerns
Many experiments were reported, but concealment and outcome-assessor blinding were not consistently established.
Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 35058751 · DOI: 10.3389/fnmol.2021.808807
Free full article in PubMed Central.
PubMed record and PubMed Central full text; extraction checked 9 August 2026.
2026-08-10