Hydrogen gas alleviates lipopolysaccharide-induced acute lung injury and inflammatory response in mice
Yin H et al. · J Inflamm (Lond). 2022;19:16.
Study at a glance
Preclinical
105 female C57BL/6 mice challenged with lipopolysaccharide and cultured mouse macrophages
Continuous 42% H₂ inhalation for 72 hours after LPS or saline administration · Final animal mixture: 42% H₂, 21% O₂ and 37% N₂. The generator output was reported as 3 L/min before nitrogen dilution.
Continuous inhalation for 72 hours
The article reports survival of 80% with LPS plus H₂ versus 60% with LPS, less tissue injury and lower several inflammatory and oxidative measures. Serum IL-6 did not change with treatment.
Mouse LPS model with continuous very-high-concentration exposure; the study was funded by the device manufacturer. Component flows are calculated from generator output, not measured inspired dose.
What kind of evidence is this?
Preclinical
Randomized mouse acute-lung-injury experiment plus RAW 264.7 macrophage experiments
Respiratory health
Inhaled H₂
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
105 female C57BL/6 mice challenged with lipopolysaccharide and cultured mouse macrophages
105 mice randomized to three groups of 35; n=10/group for 72-hour survival and the remainder for timed tissue sampling
Continuous inhalation for 72 hours
Continuous 42% H₂ inhalation for 72 hours after LPS or saline administration
Diluted oxyhydrogen: a 66.7% H₂/33.3% O₂ Brown’s-gas output was diluted with nitrogen to 42% H₂, 21% O₂ and 37% N₂.
Final animal mixture: 42% H₂, 21% O₂ and 37% N₂. The generator output was reported as 3 L/min before nitrogen dilution.
The generator’s reported 3 L/min oxyhydrogen output corresponds to about 2,010 mL/min H₂; final total chamber flow after nitrogen dilution was not reported.
The generator output corresponds to about 990 mL/min O₂ before dilution; the final animal mixture contained 21% O₂, but final O₂ flow was not reported.
LPS acute-lung-injury mice without H₂ and H₂-only controls
Outcomes and reported result
Survival, lung histology, cytokines, oxidative measures, TLR4/NF-κB signaling and macrophage-cell outcomes
The article reports survival of 80% with LPS plus H₂ versus 60% with LPS, less tissue injury and lower several inflammatory and oxidative measures. Serum IL-6 did not change with treatment.
Allocation, gas generation and composition, duration, principal positive and null results, funding and conflicts checked in the open-access full text.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Mouse LPS model with continuous very-high-concentration exposure; the study was funded by the device manufacturer. Component flows are calculated from generator output, not measured inspired dose.
LPS-induced acute lung injury in mice, not acute respiratory distress syndrome in patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36253774 · DOI: 10.1186/s12950-022-00314-x
Open-access full text is available through PMC and the publisher.
Open-access PMC/publisher full text and PubMed record; no retraction or expression of concern was shown in the checked records on 7 August 2026.
10 August 2026
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