Effect of water diuresis with hydrogen saturation on the course of acute kidney damage during the separation of oxidation and phosphorylation.
Rohovyi YY, Tsitrin VY, Bilookiy VV, Sheremet MI, Kolesnik OV. · 2022.
Study at a glance
Preclinical
Male rats with renal hypoxia induced during uncoupling of oxidation and phosphorylation.
Water with negative redox potential and 1.2 ppm dissolved molecular hydrogen. · 1.2 ppm H₂; reported redox potential −297.3±5.27 mV.
Acute experimental protocol under article-specific sampling times.
The hydrogen-saturated negative-redox water was reported to improve several renal and inflammatory laboratory measures versus the injury condition.
The intervention combines dissolved H₂ with altered redox properties, limiting attribution to H₂ alone.
What kind of evidence is this?
Preclinical
Controlled rat kidney-injury study
Kidney and urinary health
Other forms
Positive preclinical signal with water-property confounding.
Animal renal-function, enzymatic and inflammatory biomarker outcomes.
Methods
Male rats with renal hypoxia induced during uncoupling of oxidation and phosphorylation.
60 rats.
Acute experimental protocol under article-specific sampling times.
Water with negative redox potential and 1.2 ppm dissolved molecular hydrogen.
H₂ dissolved in drinking or administered water.
1.2 ppm H₂; reported redox potential −297.3±5.27 mV.
Model and water-treatment comparison groups.
Outcomes and reported result
Renal function, tubular proteinuria, enzyme activity, cytokines and protein oxidation.
The hydrogen-saturated negative-redox water was reported to improve several renal and inflammatory laboratory measures versus the injury condition.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
The intervention combines dissolved H₂ with altered redox properties, limiting attribution to H₂ alone.
The reported rat renal-hypoxia model only.
SYRCLE animal-study tool — preliminary
Some concerns
Controlled comparisons were reported, but concealment, blinding and isolation of the water's non-H₂ properties were unclear.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36567846 · DOI: 10.25122/jml-2022-0155
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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