Hydrogen-rich saline alleviates cardiomyocyte apoptosis by reducing expression of calpain1 via miR-124-3p
Xue X et al. · ESC Heart Fail. 2023;10(5):3077–3090.
Study at a glance
Preclinical
Male C57BL/6 mice and cultured neonatal rat cardiomyocytes
0.6 mmol/L hydrogen-rich saline, 10 mL/kg intraperitoneally 5 minutes before reperfusion; cells received 0.6 mmol/L H₂-rich medium · 0.6 mmol/L dissolved H₂; saline was saturated for 6 hours at 0.4 MPa and verified by gas chromatography.
30 minutes of ischemia followed by 24 hours of reperfusion; multiple cellular schedules
The article reports improved cardiac and cell-injury outcomes with H₂-rich saline and attenuation of the effect when the proposed pathway was manipulated.
Preclinical mouse and cell work, many pathway experiments and no human participants. The article declares no conflicts.
What kind of evidence is this?
Preclinical
Mouse myocardial ischemia-reperfusion experiment plus neonatal-rat cardiomyocyte experiments
Cardiovascular and circulatory health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Male C57BL/6 mice and cultured neonatal rat cardiomyocytes
50 mice divided into five groups, 10/group; five/group were used for infarct staining and five/group for stored-tissue analyses
30 minutes of ischemia followed by 24 hours of reperfusion; multiple cellular schedules
0.6 mmol/L hydrogen-rich saline, 10 mL/kg intraperitoneally 5 minutes before reperfusion; cells received 0.6 mmol/L H₂-rich medium
H₂ dissolved in saline or culture medium — H₂ only, not Brown’s gas and not inhalation.
0.6 mmol/L dissolved H₂; saline was saturated for 6 hours at 0.4 MPa and verified by gas chromatography.
Ischemia-reperfusion and hypoxia-reoxygenation controls plus pathway-manipulation groups
Outcomes and reported result
Infarct size, cardiac function, apoptosis, cell viability and miR-124-3p/calpain1 pathway
The article reports improved cardiac and cell-injury outcomes with H₂-rich saline and attenuation of the effect when the proposed pathway was manipulated.
The complete open-access article was checked for preparation, dose, animal allocation, intervention timing, figures, funding and conflicts.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical mouse and cell work, many pathway experiments and no human participants. The article declares no conflicts.
Experimental myocardial ischemia-reperfusion and cultured cardiomyocytes, not patients with myocardial infarction.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 37602925 · DOI: 10.1002/ehf2.14492
Open-access full text is available through PMC.
Open-access PMC full text and PubMed record.
10 August 2026
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