Hydrogen gas (H_2) delivered by intraperitoneal injection alleviated methionine- and choline-deficient diet-induced metabolic dysfunction–associated steatotic liver disease in mice via inhibiting GSDMD- and GSDME-mediated pyroptosis
Chen Y, Wang K, Guo W, Lu C, Suo W, Li Q, Deng Y, Chen X, Dai M, Zhang X, Xu J, Su W, Yang S, Yang H, Yan F, Liu H, Zhang Y. · Frontiers in pharmacology. 2025;16:1575106.
Study at a glance
Preclinical
cell, tissue, mice, mouse used to study metabolic and lipid health.
Full text reports H₂ dissolved in cell-culture medium plus an injected or infused H₂ intervention. Detected intervention quantities or schedules: 99.999%, 100 mL, 10 mL, 1%, 0.25 mM, 30 min. · H₂-related quantities reported in the intervention passages: 99.999%, 100 mL, 10 mL, 1%, 0.25 mM, 30 min. Values are not combined across different experimental arms.
30 min
Article-reported result excerpt (24 words maximum): “All these indicators reflecting hepatic steatosis and liver injury were improved by high dose H_2 therapy.” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
What kind of evidence is this?
Preclinical
Controlled animal and cell study
Metabolic and lipid health
Other forms
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
cell, tissue, mice, mouse used to study metabolic and lipid health.
Preclinical study; the full text reports 6 mice, with group/assay counts including n=8, n=3.
30 min
Full text reports H₂ dissolved in cell-culture medium plus an injected or infused H₂ intervention. Detected intervention quantities or schedules: 99.999%, 100 mL, 10 mL, 1%, 0.25 mM, 30 min.
H₂ dissolved in cell-culture medium plus an injected or infused H₂ intervention — H₂ only unless the full text explicitly reports oxygen co-delivery.
H₂-related quantities reported in the intervention passages: 99.999%, 100 mL, 10 mL, 1%, 0.25 mM, 30 min. Values are not combined across different experimental arms.
Control or comparison condition reported in the full article; see the linked methods for arm-specific details.
Outcomes and reported result
survival or mortality, clinical symptoms or functional scores, tumor growth or cancer markers, inflammation and cytokines, oxidative-stress and antioxidant markers, apoptosis or cell injury
Article-reported result excerpt (24 words maximum): “All these indicators reflecting hepatic steatosis and liver injury were improved by high dose H_2 therapy.” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 40860880 · DOI: 10.3389/fphar.2025.1575106
A free full article is available through PubMed Central.
Official PubMed/PMC full article; source identity, intervention data and declarations re-audited 25 August 2026.
25 August 2026
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