Hydrogenology
Hydrogenology editorial study record

Protective effect of intraportal infusion of hypothermic hydrogen-rich saline solution on hepatic warm ischemia/reperfusion injury in rat model

Golshahi H, Tavasoly A, Mardjanmehr SH, Abdi KM, Dehghan MM, Mohajeri SF. · Braz J Vet Pathol. 2017;10(1):10–21.

PreclinicalOther formsPublished 2017
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Controlled rat study

Research topic

Critical care and ischemia-reperfusion

Administration classification

Other forms

Study result signal

Positive preclinical signal, strongest for hypothermic solution.

Outcome type

Organ injury, tissue and biomarker outcomes in rats.

Reported in the source

Methods at a glance

Population or model

Wistar rats in a 70% hepatic warm ischemia/reperfusion model.

Sample

30 rats; six groups of five.

Duration

60 minutes ischemia and 120 minutes reperfusion.

Intervention

Intraportal hydrogen-rich saline at 24 °C or 4 °C ten minutes before reperfusion.

Hydrogen form

H₂ dissolved in saline.

H₂ specification

At least 0.6 mmol/L; 1 mL/kg.

H₂ flow

Not applicable.

O₂ delivered with H₂

No oxygen was co-delivered.

Comparator

Sham, ischemia/reperfusion and temperature-matched normal-saline groups.

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Histology, liver enzymes, oxidative, inflammatory, apoptotic and necrotic markers.

Reported result

The hypothermic hydrogen-rich-saline groups had lower tissue injury and multiple biochemical and inflammatory measures than ischemia/reperfusion controls.

Results-extraction completeness

The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Very small groups; temperature and H₂ are separate factors and some comparisons reflect the combined intervention.

Applies directly to

The reported acute hepatic ischemia/reperfusion model only.

Preliminary appraisal framework

SYRCLE animal-study tool — preliminary

Preliminary risk-of-bias status

Some concerns

Appraisal rationale

Multiple small groups with unclear sequence generation, concealment and blinded assessment.

Appraisal domains

Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

DOI: 10.24070/bjvp.1983-0246.v10i1p10-21 · DOI: 10.24070/bjvp.1983-0246.v10i1p10-21

Publisher access

A free publisher or repository full article was checked.

Extraction basis

Publisher or repository full article and bibliographic record; checked 10 August 2026.

Record revision

2026-08-10

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