Protective effect of intraportal infusion of hypothermic hydrogen-rich saline solution on hepatic warm ischemia/reperfusion injury in rat model
Golshahi H, Tavasoly A, Mardjanmehr SH, Abdi KM, Dehghan MM, Mohajeri SF. · Braz J Vet Pathol. 2017;10(1):10–21.
Study at a glance
Preclinical
Wistar rats in a 70% hepatic warm ischemia/reperfusion model.
Intraportal hydrogen-rich saline at 24 °C or 4 °C ten minutes before reperfusion. · At least 0.6 mmol/L; 1 mL/kg.
60 minutes ischemia and 120 minutes reperfusion.
The hypothermic hydrogen-rich-saline groups had lower tissue injury and multiple biochemical and inflammatory measures than ischemia/reperfusion controls.
Very small groups; temperature and H₂ are separate factors and some comparisons reflect the combined intervention.
What kind of evidence is this?
Preclinical
Controlled rat study
Critical care and ischemia-reperfusion
Other forms
Positive preclinical signal, strongest for hypothermic solution.
Organ injury, tissue and biomarker outcomes in rats.
Methods
Wistar rats in a 70% hepatic warm ischemia/reperfusion model.
30 rats; six groups of five.
60 minutes ischemia and 120 minutes reperfusion.
Intraportal hydrogen-rich saline at 24 °C or 4 °C ten minutes before reperfusion.
H₂ dissolved in saline.
At least 0.6 mmol/L; 1 mL/kg.
Sham, ischemia/reperfusion and temperature-matched normal-saline groups.
Outcomes and reported result
Histology, liver enzymes, oxidative, inflammatory, apoptotic and necrotic markers.
The hypothermic hydrogen-rich-saline groups had lower tissue injury and multiple biochemical and inflammatory measures than ischemia/reperfusion controls.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Very small groups; temperature and H₂ are separate factors and some comparisons reflect the combined intervention.
The reported acute hepatic ischemia/reperfusion model only.
SYRCLE animal-study tool — preliminary
Some concerns
Multiple small groups with unclear sequence generation, concealment and blinded assessment.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
DOI: 10.24070/bjvp.1983-0246.v10i1p10-21 · DOI: 10.24070/bjvp.1983-0246.v10i1p10-21
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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