Electrolyzed Reduced Water Supplemented with Platinum Nanoparticles Suppresses Promotion of Two-stage Cell Transformation.
Nishikawa R, Teruya K, Katakura Y, Osada K, Hamasaki T, Kashiwagi T, Komatsu T, Li Y, Ye J, Ichikawa A, Otsubo K, Morisawa S, Xu Q, Shirahata S. · Cytotechnology. 2005;47:97-105.
Study at a glance
Preclinical
BALB/c 3T3 cells in a two-stage transformation assay.
Electrolyzed reduced water supplemented with platinum nanoparticles. · Molecular-hydrogen exposure was not isolated from platinum nanoparticles and other water properties.
Two-stage cell-transformation protocol reported in the full article.
The platinum-supplemented electrolyzed-water condition suppressed promotion of cell-transformation foci in the reported assay.
Old in-vitro study of a multi-component intervention; it neither isolates molecular hydrogen nor demonstrates cancer prevention or treatment.
What kind of evidence is this?
Preclinical
Controlled cell-culture study
Cancer research
Other forms
Positive laboratory signal for a complex intervention; indeterminate for H2.
Cell-culture transformation and biomarker outcomes.
Methods
BALB/c 3T3 cells in a two-stage transformation assay.
Cell-culture experiments with replicate assays; no animal or human participants.
Two-stage cell-transformation protocol reported in the full article.
Electrolyzed reduced water supplemented with platinum nanoparticles.
Dissolved/active hydrogen was proposed as one property of a complex electrolyzed-water and platinum-nanoparticle intervention; not Brown's gas.
Molecular-hydrogen exposure was not isolated from platinum nanoparticles and other water properties.
Control culture medium and electrolyzed-water conditions reported in the article.
Outcomes and reported result
Transformation foci and oxidative-stress-related cell measures.
The platinum-supplemented electrolyzed-water condition suppressed promotion of cell-transformation foci in the reported assay.
The full article, exposure characterization and cell-transformation results were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Old in-vitro study of a multi-component intervention; it neither isolates molecular hydrogen nor demonstrates cancer prevention or treatment.
The reported BALB/c 3T3 cell-transformation assay only.
SYRCLE animal-study tool — preliminary
Some concerns
In-vitro complex exposure with limited reporting of blinding and selective-analysis safeguards.
Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 19003049 · DOI: 10.1007/s10616-005-3759-2
Free full article in PubMed Central.
PubMed record and PubMed Central full text; extraction checked 9 August 2026.
10 August 2026
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