Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death
Asada R, Kageyama K, Tanaka H, Matsui H, Kimura M, Saitoh Y, Miwa N. · Oncol Rep. 2010;24(6):1463–1470.
Study at a glance
Preclinical
Ehrlich ascites tumor cells in culture.
Nanobubble hydrogen-rich culture water alone or combined with platinum colloid and hyperthermia. · Approximately 1.1–1.5 ppm dissolved H₂.
24 or 48 hours at 37 °C or 42 °C.
H₂-rich medium alone reduced measured cell viability, while combinations with platinum colloid or heat were stronger.
Cell-only study; combination effects cannot be attributed to H₂ alone.
What kind of evidence is this?
Preclinical
Controlled cell-culture study
Cancer research
Other forms
Positive cell-culture signal; combinations stronger.
Cell viability and morphology outcomes.
Methods
Ehrlich ascites tumor cells in culture.
Assay replicate counts were checked in the full article.
24 or 48 hours at 37 °C or 42 °C.
Nanobubble hydrogen-rich culture water alone or combined with platinum colloid and hyperthermia.
H₂ nanobubbles dissolved in culture medium.
Approximately 1.1–1.5 ppm dissolved H₂.
Conventional medium and mineral, platinum and temperature comparison conditions.
Outcomes and reported result
Tumor-cell viability and morphology.
H₂-rich medium alone reduced measured cell viability, while combinations with platinum colloid or heat were stronger.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Cell-only study; combination effects cannot be attributed to H₂ alone.
The tested tumor-cell culture conditions only.
In-vitro study appraisal — preliminary
Some concerns
Multiple treatment combinations and assay conditions with limited reporting of replicate randomization or blinded assessment.
Independent replicate reporting checked · Exposure and comparator checked · Assay blinding generally unreported · Multiplicity and selective reporting remain possible · External validity is limited to the tested cells
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 21042740 · DOI: 10.3892/or_00001006
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
Help people check the facts before they buy.
Hydrogenology keeps 665 source-checked records accessible without advertising or product promotion.
Help us correct the recordReport a discrepancy
Send the exact difference between this page and the linked source. The study reference is attached automatically.