Hydrogenology editorial study record

Neuroprotective effect of hydrogen-rich saline in acute carbon-monoxide poisoning

Shen MH et al. · CNS Neurosci Ther. 2013;19(5):361–363.

PreclinicalOther formsPublished 2013

Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.

Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Controlled rat acute carbon-monoxide-poisoning experiment

Research topic

Other neurological conditions

Administration classification

Other forms

Study result signal

Not reported in this record.

Outcome type

Not reported in this record.

Reported in the source

Methods at a glance

Population or model

Rats exposed to acute carbon-monoxide poisoning

Sample

Principal figures report n=6–9 per group; total animal allocation is not stated as one figure

Duration

Acute treatment and tissue assessment; exact schedule requires further extraction

Intervention

Hydrogen-rich saline 10 mL/kg intraperitoneally

Hydrogen form

H₂ dissolved in saline — H₂ only, not Brown’s gas and not inhalation.

H₂ specification

Numeric dissolved-H₂ concentration is not stated in the accessible article summary.

H₂ flow

Not applicable — intraperitoneal saline, not gas inhalation.

O₂ delivered with H₂

No O₂ was co-delivered as part of the H₂ intervention.

Comparator

Carbon-monoxide-poisoned rats receiving ordinary saline and unpoisoned controls

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Neuronal survival, ROS, MDA, SOD, glutathione peroxidase, caspases, Bcl-2 and Bax

Reported result

The article reports more preserved neurons, lower ROS and MDA, higher SOD and favorable apoptosis measures. Glutathione-peroxidase activity and Bax did not significantly differ with H₂ treatment.

Results-extraction completeness

Dose, principal figures and positive and null results checked in the freely accessible PMC article.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Small preclinical poisoning study with short article format and several molecular outcomes. It does not establish treatment for human carbon-monoxide poisoning.

Applies directly to

Acute carbon-monoxide poisoning in rats.

Preliminary appraisal framework

Not reported in this record.

Preliminary risk-of-bias status

Not reported in this record.

Appraisal rationale

Not reported in this record.

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 23607699 · DOI: 10.1111/cns.12094

Publisher access

Open-access full text is available through PMC.

Extraction basis

Open-access PMC article and PubMed record.

Record revision

2026-08-10

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