Hydrogen-Rich Saline Promotes Survival of Retinal Ganglion Cells in a Rat Model of Optic Nerve Crush
Sun JC, Xu T, Zuo Q, Wang RB, Qi AQ, Cao WL, Sun AJ, Sun XJ, Xu J. · PloS one. 2014;9(6):e99299.
What kind of evidence is this?
Preclinical
Controlled animal and cell study
Eye health
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
rats, cell, cells, rabbit used to study eye health.
Preclinical study; the article does not state one consolidated sample total, so no total is inferred.
2 weeks
Full text reports H₂ dissolved in saline. Detected intervention quantities or schedules: 5 ml/kg, 2 weeks.
H₂ dissolved in saline — H₂ only unless the full text explicitly reports oxygen co-delivery.
H₂-related quantities reported in the intervention passages: 5 ml/kg, 2 weeks. Values are not combined across different experimental arms.
Not applicable — no inhaled H₂ intervention was identified in the full article.
No inhaled O₂ co-delivery was identified for this non-inhalation intervention.
Placebo or matched control reported in the full article.
Outcomes and reported result
survival or mortality, clinical symptoms or functional scores, tumor growth or cancer markers, oxidative-stress and antioxidant markers, apoptosis or cell injury, metabolic laboratory measures
Article-reported result excerpt (24 words maximum): “There is no difference between sham group and H_2 group (P = 0.246).” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. No funding statement was identified in the machine-readable full article; absence is not inferred. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
Applies to the reported animal, cell or tissue model, not directly to patients.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 24915536 · DOI: 10.1371/journal.pone.0099299
A free full article is available through PubMed Central.
Official Europe PMC JATS full article and PubMed/PMC identifiers; structured full-text extraction and validation completed 8 August 2026.
2026-08-10