Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water
Hamasaki T, Harada G, Nakamichi N, Kabayama S, Teruya K, Fugetsu B, Gong W, Sakata I, Shirahata S. · PloS one. 2017;12(2):e0171192.
Study at a glance
Preclinical
cell, cells used to study other molecular hydrogen research.
Full text reports H₂ dissolved in drinking water. Detected intervention quantities or schedules: 40 mM, 30 min, 99.7%, 1000 ppm, 10 ppm, 99.999%, 2 ppm, 1 ppm, 5 ppm, 99.998%. · H₂-related quantities reported in the intervention passages: 40 mM, 30 min, 99.7%, 1000 ppm, 10 ppm, 99.999%, 2 ppm, 1 ppm, 5 ppm, 99.998%. Values are not combined across different experimental arms.
30 min
Article-reported result excerpt (24 words maximum): “The FL intensity was found to increase only when treated with 60 μM of hydrogen peroxide for 30 min ([Fig 1], right).” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
What kind of evidence is this?
Preclinical
Controlled cell or tissue study
Other molecular hydrogen research
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
cell, cells used to study other molecular hydrogen research.
Preclinical study; the article does not state one consolidated sample total, so no total is inferred.
30 min
Full text reports H₂ dissolved in drinking water. Detected intervention quantities or schedules: 40 mM, 30 min, 99.7%, 1000 ppm, 10 ppm, 99.999%, 2 ppm, 1 ppm, 5 ppm, 99.998%.
H₂ dissolved in drinking water — H₂ only unless the full text explicitly reports oxygen co-delivery.
H₂-related quantities reported in the intervention passages: 40 mM, 30 min, 99.7%, 1000 ppm, 10 ppm, 99.999%, 2 ppm, 1 ppm, 5 ppm, 99.998%. Values are not combined across different experimental arms.
Control or comparison condition reported in the full article; see the linked methods for arm-specific details.
Outcomes and reported result
oxidative-stress and antioxidant markers, apoptosis or cell injury
Article-reported result excerpt (24 words maximum): “The FL intensity was found to increase only when treated with 60 μM of hydrogen peroxide for 30 min ([Fig 1], right).” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 28182635 · DOI: 10.1371/journal.pone.0171192
A free full article is available through PubMed Central.
Official PubMed/PMC full article; source identity, intervention data and declarations re-audited 25 August 2026.
25 August 2026
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