Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem Cell Injury by Reducing Hydroxyl Radical
Zhang J et al. · Oxid Med Cell Longev. 2017;2017:8241678.
Study at a glance
Preclinical
C57BL mice exposed to sublethal or lethal total-body irradiation
0.5 mL hydrogen-rich water by gavage 10 minutes before irradiation and daily for 7 days afterward · Fresh water was maintained above 0.8 ppm dissolved H₂ (reported as about 1 ppm); H₂ gas was bubbled into 500 mL water at 150 mL/min for 20 minutes.
One pretreatment plus 7 days after irradiation; survival followed for 30 days and selected stem-cell outcomes longer
The article reports higher survival in lethal-dose cohorts and favorable hematopoietic-stem-cell, oxidative-stress and DNA-damage measures with H₂ water.
Preclinical radiation model with many laboratory outcomes, several cohort sizes and no human participants. Authors declared no competing interests.
What kind of evidence is this?
Preclinical
Controlled mouse total-body-irradiation survival and hematopoietic-stem-cell experiments
Cancer research
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
C57BL mice exposed to sublethal or lethal total-body irradiation
Survival cohorts n=15/group at 6.8 Gy and n=18/group at 7.2 Gy; laboratory cohorts commonly n=3–8/group
One pretreatment plus 7 days after irradiation; survival followed for 30 days and selected stem-cell outcomes longer
0.5 mL hydrogen-rich water by gavage 10 minutes before irradiation and daily for 7 days afterward
H₂ dissolved in water — H₂ only, not Brown’s gas and not inhalation.
Fresh water was maintained above 0.8 ppm dissolved H₂ (reported as about 1 ppm); H₂ gas was bubbled into 500 mL water at 150 mL/min for 20 minutes.
Irradiated mice receiving vehicle water and non-irradiated controls
Outcomes and reported result
Survival, blood-cell differentiation, stem and progenitor cells, self-renewal, ROS, DNA damage, apoptosis and antioxidant proteins
The article reports higher survival in lethal-dose cohorts and favorable hematopoietic-stem-cell, oxidative-stress and DNA-damage measures with H₂ water.
The complete open-access article was checked for water preparation, concentration, allocation, schedule, figures, principal results and conflicts.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical radiation model with many laboratory outcomes, several cohort sizes and no human participants. Authors declared no competing interests.
Total-body irradiation in mice, not prevention of treatment toxicity in people.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 28243358 · DOI: 10.1155/2017/8241678
Open-access full text is available through PMC.
Open-access PMC full text and PubMed record.
10 August 2026
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