Antioxidants Attenuate Oxidative Stress-Induced Hidden Blood Loss in Rats.
Qian H, Yuan T, Tong J, Sun WS, Jin J, Chen WX, Meng J, Bao N, Zhao J. · 2017.
Study at a glance
Preclinical
Rats in a linoleic-acid oxidative-stress model of hidden blood loss.
Hydrogen water or proanthocyanidin after model induction. · Article-specific preparation and dosing schedule.
Acute experimental protocol.
Hydrogen water and proanthocyanidin were each reported to attenuate several blood-count and oxidative-stress changes versus the model condition.
Animal model with laboratory surrogates; the comparator antioxidant does not validate clinical prevention of blood loss.
What kind of evidence is this?
Preclinical
Controlled rat hidden-blood-loss study
Critical care and ischemia-reperfusion
H₂-rich water
Positive preclinical signal.
Animal hematologic and oxidative-stress outcomes.
Methods
Rats in a linoleic-acid oxidative-stress model of hidden blood loss.
Animal groups and assay counts were checked in the full article.
Acute experimental protocol.
Hydrogen water or proanthocyanidin after model induction.
H₂ dissolved in water.
Article-specific preparation and dosing schedule.
Model controls and a separate proanthocyanidin antioxidant arm.
Outcomes and reported result
Hemoglobin, red-cell count, antioxidant enzymes, malondialdehyde and ferryl hemoglobin.
Hydrogen water and proanthocyanidin were each reported to attenuate several blood-count and oxidative-stress changes versus the model condition.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal model with laboratory surrogates; the comparator antioxidant does not validate clinical prevention of blood loss.
The reported rat oxidative hidden-blood-loss model only.
SYRCLE animal-study tool — preliminary
Some concerns
Controlled groups were used, but concealment, blinded assays and multiplicity handling were unclear.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 28270376 · DOI: 10.4274/tjh.2016.0469
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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