Hydrogen ameliorates oxidative stress via PI3K-Akt signaling pathway in UVB-induced HaCaT cells
Zhang B, Zhao Z, Meng X, Chen H, Fu G, Xie K. · Int J Mol Med. 2018;41(6):3653–3661.
Study at a glance
Preclinical
HaCaT keratinocytes exposed to UVB radiation.
Hydrogen-rich culture medium after UVB exposure, with a PI3K inhibitor in mechanistic experiments. · 0.6 mmol/L dissolved H₂.
Up to 24 hours depending on assay.
Several viability, oxidative, apoptotic and pathway measures favored hydrogen-rich medium after UVB exposure.
Cell-only evidence; pathway-inhibitor experiments are mechanistic and do not establish patient benefit.
What kind of evidence is this?
Preclinical
Controlled cell-culture study
Skin, wound and aging research
Other forms
Positive cell-culture signal.
Cell viability and mechanistic pathway outcomes.
Methods
HaCaT keratinocytes exposed to UVB radiation.
Approximately five to six independent observations per assay as reported.
Up to 24 hours depending on assay.
Hydrogen-rich culture medium after UVB exposure, with a PI3K inhibitor in mechanistic experiments.
H₂ dissolved in cell-culture medium.
0.6 mmol/L dissolved H₂.
UVB-exposed and non-exposed conventional medium, with pathway-inhibitor comparisons.
Outcomes and reported result
Cell viability, ROS, oxidative markers, apoptosis and PI3K/Akt/FoxO signaling.
Several viability, oxidative, apoptotic and pathway measures favored hydrogen-rich medium after UVB exposure.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Cell-only evidence; pathway-inhibitor experiments are mechanistic and do not establish patient benefit.
The HaCaT cell model and tested exposure only.
In-vitro study appraisal — preliminary
Some concerns
Assay comparators were present, but replicate-level randomization, blinding and multiplicity control were not established.
Independent replicate reporting checked · Exposure and comparator checked · Assay blinding generally unreported · Multiplicity and selective reporting remain possible · External validity is limited to the tested cells
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 29532858 · DOI: 10.3892/ijmm.2018.3550
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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