Hydrogenology
Hydrogenology editorial study record

Efficacy of silicon-based agent against aging-related frailty.

Koyama Y, Kobayashi Y, Kobayashi H, Shimada S. · Scientific Reports. 2026;16:39711.

PreclinicalOther formsPublished 2026
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Controlled mouse studies

Research topic

Skin, wound and aging research

Administration classification

Other forms

Study result signal

Mixed preclinical signal.

Outcome type

Animal functional and survival outcomes.

Reported in the source

Methods at a glance

Population or model

Klotho-deficient mice and very old male C57BL/6J mice.

Sample

Klotho experiment began with 20 mice per group; aged-mouse batches included 21 control and 20 supplemented mice.

Duration

Three weeks in the klotho model and approximately nine weeks in aged mice.

Intervention

Diet containing 1.0% or 2.5% silicon-based hydrogen-generating agent, depending on experiment.

Hydrogen form

Hydrogen generated in the gastrointestinal tract by a silicon-based dietary agent; not Brown's gas.

H₂ specification

A directly administered molecular-H2 dose was not measured.

H₂ flow

Not applicable — dietary agent.

O₂ delivered with H₂

No oxygen was co-delivered.

Comparator

Matched control diet.

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Frailty-related behavior, balance, body weight and survival.

Reported result

Several frailty and functional measures favored supplementation; the full-period survival comparison was not statistically significant.

Results-extraction completeness

The full article, both animal models, figures, exclusions and significant and null survival findings were checked.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Animal evidence only. Some deaths occurred before behavioral testing, and silicon-product effects cannot be assigned exclusively to hydrogen.

Applies directly to

The two reported mouse aging models.

Preliminary appraisal framework

SYRCLE animal-study tool — preliminary

Preliminary risk-of-bias status

Some concerns

Appraisal rationale

Deaths before testing and incomplete reporting of concealment and assessor blinding create attrition and measurement concerns.

Appraisal domains

Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 41688516 · DOI: 10.1038/s41598-026-39711-0

Publisher access

Free full article in PubMed Central.

Extraction basis

PubMed record and PubMed Central full text; extraction checked 9 August 2026.

Record revision

2026-08-10