Efficacy of silicon-based agent against aging-related frailty.
Koyama Y, Kobayashi Y, Kobayashi H, Shimada S. · Scientific Reports. 2026;16:39711.
Study at a glance
Preclinical
Klotho-deficient mice and very old male C57BL/6J mice.
Diet containing 1.0% or 2.5% silicon-based hydrogen-generating agent, depending on experiment. · A directly administered molecular-H2 dose was not measured.
Three weeks in the klotho model and approximately nine weeks in aged mice.
Several frailty and functional measures favored supplementation; the full-period survival comparison was not statistically significant.
Animal evidence only. Some deaths occurred before behavioral testing, and silicon-product effects cannot be assigned exclusively to hydrogen.
What kind of evidence is this?
Preclinical
Controlled mouse studies
Skin, wound and aging research
Other forms
Mixed preclinical signal.
Animal functional and survival outcomes.
Methods
Klotho-deficient mice and very old male C57BL/6J mice.
Klotho experiment began with 20 mice per group; aged-mouse batches included 21 control and 20 supplemented mice.
Three weeks in the klotho model and approximately nine weeks in aged mice.
Diet containing 1.0% or 2.5% silicon-based hydrogen-generating agent, depending on experiment.
Hydrogen generated in the gastrointestinal tract by a silicon-based dietary agent; not Brown's gas.
A directly administered molecular-H2 dose was not measured.
Matched control diet.
Outcomes and reported result
Frailty-related behavior, balance, body weight and survival.
Several frailty and functional measures favored supplementation; the full-period survival comparison was not statistically significant.
The full article, both animal models, figures, exclusions and significant and null survival findings were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal evidence only. Some deaths occurred before behavioral testing, and silicon-product effects cannot be assigned exclusively to hydrogen.
The two reported mouse aging models.
SYRCLE animal-study tool — preliminary
Some concerns
Deaths before testing and incomplete reporting of concealment and assessor blinding create attrition and measurement concerns.
Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 41688516 · DOI: 10.1038/s41598-026-39711-0
Free full article in PubMed Central.
PubMed record and PubMed Central full text; extraction checked 9 August 2026.
10 August 2026
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