Hydrogenology
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Hydrogen-rich water ameliorates rat placental stress induced by water restriction

Mano Y et al. · Med Gas Res. 2018;8(3):79–84.

PreclinicalH₂-rich waterPublished 2018
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Controlled three-group pregnancy experiment

Research topic

Women’s health

Administration classification

H₂-rich water

Study result signal

Not reported in this record.

Outcome type

Not reported in this record.

Reported in the source

Methods at a glance

Population or model

Pregnant Wistar rats exposed to restricted water access

Sample

36 pregnant rats; control n=12, water-restriction n=12 and H₂-water n=12

Duration

Gestational days 1–17 under the reported drinking protocol

Intervention

Hydrogen-rich water twice daily on days 1–6, followed by one hour/day access during gestational days 7–17

Hydrogen form

H₂ dissolved in drinking water — H₂ only, not Brown’s gas.

H₂ specification

500–800 µg/L dissolved H₂.

H₂ flow

Not applicable — oral water, not gas inhalation.

O₂ delivered with H₂

No O₂ was co-delivered as part of the intervention.

Comparator

Unrestricted control and water-restricted group receiving ordinary water

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Fetal and placental measurements, serum osmolality and oxidative stress, placental histology and inflammatory or oxidative-stress biomarkers

Reported result

Several placental histology and biomarker findings improved with H₂ water, but fetal weight and crown-rump length remained lower than unrestricted controls and fetal weight did not differ significantly from ordinary-water restriction.

Results-extraction completeness

Full methods and principal positive and null findings checked in the free full text; independent appraisal remains incomplete.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Animal pregnancy model with an unusual restricted-drinking protocol and biomarker-heavy outcomes. It cannot establish safety or benefit during human pregnancy.

Applies directly to

Pregnant rats under water restriction; not pregnant people.

Preliminary appraisal framework

Not reported in this record.

Preliminary risk-of-bias status

Not reported in this record.

Appraisal rationale

Not reported in this record.

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 30319761 · DOI: 10.4103/2045-9912.241064

Publisher access

Open-access full text is available through PMC.

Extraction basis

Open-access PMC full text and PubMed bibliographic record.

Record revision

2026-08-10

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