Interaction between gender and post resuscitation interventions on neurological outcome in an asphyxial rat model of cardiac arrest.
Wang J, Li J, Chen B, Shen Y, Wang J, Wang K, Yin C, Li Y. · 2021.
Study at a glance
Preclinical
Male and female rats after asphyxial cardiac arrest and resuscitation.
2% H₂ with 98% O₂ for one hour after resuscitation under normothermia. · 2% H₂ and 98% O₂ for one hour.
One-hour gas intervention with 96-hour neurological and survival follow-up.
Female rats in the hydrogen arm had better selected neurological and survival measures than males; the study emphasizes an interaction rather than an overall clinical effect.
Animal model, subgroup/interaction interpretation and several comparisons; does not establish sex-specific clinical efficacy.
What kind of evidence is this?
Preclinical
Randomized controlled rat cardiac-arrest study
Critical care and ischemia-reperfusion
Inhaled H₂
Sex-interaction exploratory signal.
Animal survival and neurological functional outcomes.
Methods
Male and female rats after asphyxial cardiac arrest and resuscitation.
120 rats: 60 male and 60 female; three post-resuscitation groups of 40.
One-hour gas intervention with 96-hour neurological and survival follow-up.
2% H₂ with 98% O₂ for one hour after resuscitation under normothermia.
Inhaled molecular H₂ with oxygen.
2% H₂ and 98% O₂ for one hour.
Absolute gas flow was not reported in the abstract and is not inferred.
98% O₂ was co-delivered.
Normothermic control and targeted-temperature-management groups using 2% N₂/98% O₂.
Outcomes and reported result
Neurological deficit, electroencephalographic recovery and survival, stratified by sex.
Female rats in the hydrogen arm had better selected neurological and survival measures than males; the study emphasizes an interaction rather than an overall clinical effect.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal model, subgroup/interaction interpretation and several comparisons; does not establish sex-specific clinical efficacy.
The reported asphyxial cardiac-arrest rat model only.
SYRCLE animal-study tool — preliminary
Some concerns
Randomization was reported, while concealment, blinded assessment and prespecification of interaction analyses were unclear.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 34530726 · DOI: 10.1186/s12872-021-02262-5
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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