Hydrogen-rich water exerts anti-tumor effects comparable to 5-fluorouracil in a colorectal cancer xenograft model
Asgharzadeh F, Tarnava A, Mostafapour A, Khazaei M, LeBaron TW. · World journal of gastrointestinal oncology. 2022;14(1):242-252.
What kind of evidence is this?
Preclinical
Controlled animal and cell study
Cancer research
H₂-rich water
Not reported in this record.
Not reported in this record.
Methods at a glance
cell, mouse, cells, mice used to study cancer research.
Preclinical study; group or assay counts reported in the full text include n=6.
Multiple article-specific time points; see the linked full methods.
Full text reports H₂ dissolved in drinking water. No numeric quantity is inferred beyond the source wording.
H₂ dissolved in drinking water — H₂ only unless the full text explicitly reports oxygen co-delivery.
The full article describes the H₂ intervention but does not report a numeric H₂ concentration.
Not applicable — no inhaled H₂ intervention was identified in the full article.
No inhaled O₂ co-delivery was identified for this non-inhalation intervention.
Control or comparison condition reported in the full article; see the linked methods for arm-specific details.
Outcomes and reported result
survival or mortality, clinical symptoms or functional scores, tumor growth or cancer markers, oxidative-stress and antioxidant markers, apoptosis or cell injury, histology or tissue damage
Article-reported result excerpt (24 words maximum): “In contrast, with both H_2 and 5-FU alone, the percentage of collagen deposition in the tumor tissue was significantly reduced to about 13% (…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. No funding statement was identified in the machine-readable full article; absence is not inferred. The article declares no conflicts of interest.
Applies to the reported animal, cell or tissue model, not directly to patients.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 35116114 · DOI: 10.4251/wjgo.v14.i1.242
A free full article is available through PubMed Central.
Official Europe PMC JATS full article and PubMed/PMC identifiers; structured full-text extraction and validation completed 8 August 2026.
2026-08-10