Nano-bubble hydrogen water: An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model
Li C, Cao Y, Kohei F, Hao H, Peng G, Cheng C, Ye J. · Virologica Sinica. 2022;37(2):277-283.
Study at a glance
Preclinical
Three-month-old zebrafish infected with spring viraemia of carp virus, with additional ZF4-cell experiments.
SVCV-infected zebrafish were kept in nano-bubble H₂ water; parallel cell experiments used nano-H₂ water-treated medium. · Nano-bubble H₂ water concentration: approximately 0.7 ppm.
See the linked full methods for assay-specific exposure and follow-up schedules.
Article-reported result excerpt (24 words maximum): “However, in aquaculture water rearing zebrafish, hydrogen nano-bubbles reduced more quickly.” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
What kind of evidence is this?
Preclinical
Controlled cell or tissue study
Oral and dental health
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Three-month-old zebrafish infected with spring viraemia of carp virus, with additional ZF4-cell experiments.
Preclinical zebrafish and cell study; no single consolidated sample total is stated in the article.
See the linked full methods for assay-specific exposure and follow-up schedules.
SVCV-infected zebrafish were kept in nano-bubble H₂ water; parallel cell experiments used nano-H₂ water-treated medium.
H₂ dissolved in drinking water — H₂ only unless the full text explicitly reports oxygen co-delivery.
Nano-bubble H₂ water concentration: approximately 0.7 ppm.
SVCV-infected zebrafish kept in ordinary aquaculture water and corresponding untreated cell controls.
Outcomes and reported result
Mortality, viral replication, histopathology, reactive oxygen species and inflammatory cytokines in zebrafish, plus cell assays.
Article-reported result excerpt (24 words maximum): “However, in aquaculture water rearing zebrafish, hydrogen nano-bubbles reduced more quickly.” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 35249853 · DOI: 10.1016/j.virs.2022.01.023
A free full article is available through PubMed Central.
Official PubMed/PMC full article; record re-audited and corrected 25 August 2026.
25 August 2026
Help people check the facts before they buy.
Hydrogenology keeps 665 source-checked records accessible without advertising or product promotion.
Help us correct the recordReport a discrepancy
Send the exact difference between this page and the linked source. The study reference is attached automatically.