Hydrogen-generating Si-based agent protects against skin flap ischemia-reperfusion injury in rats.
Otani N, Tomita K, Kobayashi Y, Kuroda K, Koyama Y, Kobayashi H, Kubo T. · Scientific Reports. 2022;12:10228.
Study at a glance
Preclinical
Adult male Sprague-Dawley rats in a skin-flap ischemia-reperfusion model.
AIN93M diet containing 1.0% silicon-based hydrogen-generating agent from one week before surgery. · A directly administered molecular-H2 dose was not measured.
One week of pretreatment, then 72 hours of reperfusion follow-up.
Flap survival was higher in supplemented rats than in ischemia-reperfusion controls, with favorable tissue and oxidative-stress measures.
Animal evidence only; the silicon-based product is not equivalent to a clinical molecular-hydrogen intervention.
What kind of evidence is this?
Preclinical
Controlled rat study
Skin, wound and aging research
Other forms
Positive preclinical signal.
Animal tissue, perfusion and functional survival outcomes.
Methods
Adult male Sprague-Dawley rats in a skin-flap ischemia-reperfusion model.
24 rats; 8 each in sham, ischemia-reperfusion and supplemented ischemia-reperfusion groups.
One week of pretreatment, then 72 hours of reperfusion follow-up.
AIN93M diet containing 1.0% silicon-based hydrogen-generating agent from one week before surgery.
Hydrogen generated in the gastrointestinal tract by a silicon-based dietary agent; not Brown's gas.
A directly administered molecular-H2 dose was not measured.
Sham and ischemia-reperfusion groups on control diet.
Outcomes and reported result
Skin-flap survival, blood flow, histology and oxidative-stress measures.
Flap survival was higher in supplemented rats than in ischemia-reperfusion controls, with favorable tissue and oxidative-stress measures.
The full article, methods, figures and measured outcomes were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Animal evidence only; the silicon-based product is not equivalent to a clinical molecular-hydrogen intervention.
The reported rat skin-flap model.
SYRCLE animal-study tool — preliminary
Some concerns
Small groups and unclear allocation concealment and assessor blinding.
Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 35418596 · DOI: 10.1038/s41598-022-10228-6
Free full article in PubMed Central.
PubMed record and PubMed Central full text; extraction checked 9 August 2026.
10 August 2026
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