Hydrogen-generating Si-based agent protects against skin flap ischemia-reperfusion injury in rats.
Otani N, Tomita K, Kobayashi Y, Kuroda K, Koyama Y, Kobayashi H, Kubo T. · Scientific Reports. 2022;12:10228.
What kind of evidence is this?
Preclinical
Controlled rat study
Skin, wound and aging research
Other forms
Positive preclinical signal.
Animal tissue, perfusion and functional survival outcomes.
Methods at a glance
Adult male Sprague-Dawley rats in a skin-flap ischemia-reperfusion model.
24 rats; 8 each in sham, ischemia-reperfusion and supplemented ischemia-reperfusion groups.
One week of pretreatment, then 72 hours of reperfusion follow-up.
AIN93M diet containing 1.0% silicon-based hydrogen-generating agent from one week before surgery.
Hydrogen generated in the gastrointestinal tract by a silicon-based dietary agent; not Brown's gas.
A directly administered molecular-H2 dose was not measured.
Not applicable — dietary agent.
No oxygen was co-delivered.
Sham and ischemia-reperfusion groups on control diet.
Outcomes and reported result
Skin-flap survival, blood flow, histology and oxidative-stress measures.
Flap survival was higher in supplemented rats than in ischemia-reperfusion controls, with favorable tissue and oxidative-stress measures.
The full article, methods, figures and measured outcomes were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Animal evidence only; the silicon-based product is not equivalent to a clinical molecular-hydrogen intervention.
The reported rat skin-flap model.
SYRCLE animal-study tool — preliminary
Some concerns
Small groups and unclear allocation concealment and assessor blinding.
Randomization/allocation reporting is limited · Blinding of personnel is unclear · Attrition is incompletely reported in places · Blinding of outcome assessors is unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 35418596 · DOI: 10.1038/s41598-022-10228-6
Free full article in PubMed Central.
PubMed record and PubMed Central full text; extraction checked 9 August 2026.
2026-08-10