H 2 -induced transient upregulation of phospholipids with suppression of energy metabolism.
Iketani M, Sakane I, Fujita Y, Ito M, Ohsawa I. · 2023.
Study at a glance
Preclinical
SH-SY5Y neuroblastoma cells exposed to molecular hydrogen.
One-hour molecular-hydrogen exposure under the article's cell-culture gas protocol. · The full article reports the gas-exposure preparation; no systemic dose applies.
One-hour exposure with time-course measurements.
The article reports transient phospholipid increases, suppressed energy metabolism, lower glutathione and delayed endosomal transport after H₂ exposure.
Cell-only mechanistic study; several changes were transient and some reflected increased rather than reduced stress.
What kind of evidence is this?
Preclinical
Controlled in-vitro neuroblastoma-cell study
Other molecular hydrogen research
Other forms
Mixed transient mechanistic signal.
Cell lipidomic, metabolic and trafficking outcomes.
Methods
SH-SY5Y neuroblastoma cells exposed to molecular hydrogen.
Cell-culture assays with replicate counts reported by experiment.
One-hour exposure with time-course measurements.
One-hour molecular-hydrogen exposure under the article's cell-culture gas protocol.
Molecular H₂ exposure of cell cultures.
The full article reports the gas-exposure preparation; no systemic dose applies.
Unexposed control cultures and serial post-exposure time points.
Outcomes and reported result
Lipidomics, metabolomics, glutathione, energy metabolism and endosomal transport.
The article reports transient phospholipid increases, suppressed energy metabolism, lower glutathione and delayed endosomal transport after H₂ exposure.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Cell-only mechanistic study; several changes were transient and some reflected increased rather than reduced stress.
The tested SH-SY5Y cell system only.
In-vitro study appraisal — preliminary
Some concerns
Controlled cultures were used, but replicate independence, assay blinding and high-dimensional multiple testing remain concerns.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36571379 · DOI: 10.4103/2045-9912.344973
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
10 August 2026
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