H 2 -induced transient upregulation of phospholipids with suppression of energy metabolism.
Iketani M, Sakane I, Fujita Y, Ito M, Ohsawa I. · 2023.
What kind of evidence is this?
Preclinical
Controlled in-vitro neuroblastoma-cell study
Other molecular hydrogen research
Other forms
Mixed transient mechanistic signal.
Cell lipidomic, metabolic and trafficking outcomes.
Methods at a glance
SH-SY5Y neuroblastoma cells exposed to molecular hydrogen.
Cell-culture assays with replicate counts reported by experiment.
One-hour exposure with time-course measurements.
One-hour molecular-hydrogen exposure under the article's cell-culture gas protocol.
Molecular H₂ exposure of cell cultures.
The full article reports the gas-exposure preparation; no systemic dose applies.
Not applicable to patient inhalation; laboratory gas exposure only.
Cell-culture gas conditions are reported in the methods.
Unexposed control cultures and serial post-exposure time points.
Outcomes and reported result
Lipidomics, metabolomics, glutathione, energy metabolism and endosomal transport.
The article reports transient phospholipid increases, suppressed energy metabolism, lower glutathione and delayed endosomal transport after H₂ exposure.
The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Cell-only mechanistic study; several changes were transient and some reflected increased rather than reduced stress.
The tested SH-SY5Y cell system only.
In-vitro study appraisal — preliminary
Some concerns
Controlled cultures were used, but replicate independence, assay blinding and high-dimensional multiple testing remain concerns.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 36571379 · DOI: 10.4103/2045-9912.344973
A free full article is available through PubMed Central.
Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.
2026-08-10