Impact of hydrogen gas inhalation during therapeutic hypothermia on cerebral hemodynamics and oxygenation in the asphyxiated piglet
Nakamura S, Nakao Y, Htun Y, Mitsuie T, Koyano K, Morimoto A, Konishi Y, Arioka M, Kondo S, Kato I, Ohta KI, Yasuda S, Miki T, Ueno M, Kusaka T. · Scientific reports. 2023;13(1):1615.
Study at a glance
Preclinical
Asphyxiated newborn piglets studied during normothermia or therapeutic hypothermia.
Ventilator-delivered H₂ for 24 hours during therapeutic hypothermia. The inhaled H₂ concentration varied from 2.1% to 2.7% as oxygen requirements changed. · Delivered H₂: usually 2.1–2.7%; FiO₂ range 0.21–0.40. The source cylinder contained 3.8% H₂ and 96.2% N₂; 3.8% was not the final delivered concentration.
24 hours of H₂ ventilation during therapeutic hypothermia.
Article-reported result excerpt (24 words maximum): “The duration of LAEEG after the insult did not differ among the groups [mean (SD): NT group, 25.4 (8.0) min; TH group, 26.2 (13.2)…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
What kind of evidence is this?
Preclinical
Controlled animal and cell study
Other molecular hydrogen research
Inhaled H₂
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Asphyxiated newborn piglets studied during normothermia or therapeutic hypothermia.
26 piglets: normothermia n=10, hypothermia n=8 and hypothermia plus H₂ n=8.
24 hours of H₂ ventilation during therapeutic hypothermia.
Ventilator-delivered H₂ for 24 hours during therapeutic hypothermia. The inhaled H₂ concentration varied from 2.1% to 2.7% as oxygen requirements changed.
Inhaled H₂ prepared from a 3.8% H₂/96.2% N₂ cylinder with supplemental O₂; not Brown’s gas.
Delivered H₂: usually 2.1–2.7%; FiO₂ range 0.21–0.40. The source cylinder contained 3.8% H₂ and 96.2% N₂; 3.8% was not the final delivered concentration.
Not reported in the full article — an absolute H₂ flow in mL/min could not be verified.
O₂ was adjusted according to each piglet’s need (FiO₂ 0.21–0.40); absolute O₂ flow was not reported.
Asphyxia with normothermia and asphyxia with therapeutic hypothermia without H₂.
Outcomes and reported result
survival or mortality, clinical symptoms or functional scores, oxidative-stress and antioxidant markers, apoptosis or cell injury, histology or tissue damage, metabolic laboratory measures
Article-reported result excerpt (24 words maximum): “The duration of LAEEG after the insult did not differ among the groups [mean (SD): NT group, 25.4 (8.0) min; TH group, 26.2 (13.2)…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 36709361 · DOI: 10.1038/s41598-023-28274-z
A free full article is available through PubMed Central.
Official PubMed/PMC full article; record re-audited and corrected 25 August 2026.
25 August 2026
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