Molecular Hydrogen Affords Similar Neuroprotection to Therapeutic Hypothermia in a Porcine Model of Neonatal Hypoxic–Ischemic Encephalopathy
Balog E, Remzső G, Tóth-Szűki V, Rózsa É, Kovács V, Domoki F. · Antioxidants (Basel, Switzerland). 2025;14(12):1405.
Study at a glance
Preclinical
Twenty-two newborn male Landrace piglets in a hypoxic–ischemic encephalopathy model.
After 20 minutes of induced asphyxia, the H₂ group was continuously ventilated with 2.1% H₂, 21% O₂ and 76.9% N₂ during the 48-hour observation period. · 2.1% H₂, 21% O₂ and 76.9% N₂. Absolute gas flow was not reported.
Continuous ventilation during 48 hours of post-asphyxia observation.
Article-reported result excerpt (24 words maximum): “The total duration of seizure activity did not differ significantly between the A-NT (3.4 ± 1.0 h) and A-H_2 (3.1 ± 0.7 h) groups…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
What kind of evidence is this?
Preclinical
Controlled preclinical study
Other molecular hydrogen research
Inhaled H₂
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Twenty-two newborn male Landrace piglets in a hypoxic–ischemic encephalopathy model.
22 piglets: asphyxia-normothermia n=6, asphyxia-H₂ n=8 and asphyxia-hypothermia n=8.
Continuous ventilation during 48 hours of post-asphyxia observation.
After 20 minutes of induced asphyxia, the H₂ group was continuously ventilated with 2.1% H₂, 21% O₂ and 76.9% N₂ during the 48-hour observation period.
Inhaled H₂/N₂/O₂ research mixture — 2.1% H₂, not Brown’s gas.
2.1% H₂, 21% O₂ and 76.9% N₂. Absolute gas flow was not reported.
Not reported — the source gives gas percentages but no total flow.
21% O₂; absolute O₂ flow was not reported.
Asphyxia with normothermia and asphyxia with therapeutic hypothermia.
Outcomes and reported result
Physiological measures, EEG/seizures, visual evoked potentials and neuropathology.
Article-reported result excerpt (24 words maximum): “The total duration of seizure activity did not differ significantly between the A-NT (3.4 ± 1.0 h) and A-H_2 (3.1 ± 0.7 h) groups…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 41462605 · DOI: 10.3390/antiox14121405
A free full article is available through PubMed Central.
Official PubMed/PMC full article; record re-audited and corrected 25 August 2026.
25 August 2026
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