Hydrogenology
Hydrogenology editorial study record

Hydrogen Promotes the Effectiveness of Bone Mesenchymal Stem Cell Transplantation in Rats with Spinal Cord Injury

Luo S, Wu J, Qiu Y, Xiao B, Xi Y, Yang C, Shi Z, Wang W. · 2023.

PreclinicalOther formsPublished 2023
Study record, not medical adviceThis page reports one source and Hydrogenology’s source-text extraction. It does not establish that molecular hydrogen is effective, safe or appropriate for any person. Human, animal and laboratory evidence must not be treated as equivalent.
Source and classification

What kind of evidence is this?

Evidence stream

Preclinical

Reported design

Controlled rat and cell study

Research topic

Other neurological conditions

Administration classification

Other forms

Study result signal

Positive preclinical signal; combination arm emphasized.

Outcome type

Animal functional, tissue and cell-mechanistic outcomes.

Reported in the source

Methods at a glance

Population or model

Rats with experimental spinal-cord injury and cultured bone-marrow mesenchymal stem cells.

Sample

Animal and cell groups were checked in the full article; assay-specific counts vary.

Duration

Repeated treatment with follow-up through 28 days.

Intervention

Hydrogen-rich saline with or without bone-marrow mesenchymal stem-cell transplantation.

Hydrogen form

H₂ dissolved in saline.

H₂ specification

0.6 mmol/L; 5 mL/kg at the reported repeated schedule.

H₂ flow

Not applicable.

O₂ delivered with H₂

No oxygen was co-delivered.

Comparator

Injury controls, hydrogen-saline and stem-cell treatment arms.

Reported, not endorsed

Outcomes and reported result

Outcomes measured

Locomotor function, spinal histology, cell survival and molecular markers.

Reported result

The combined hydrogen-saline and stem-cell condition was reported to improve cell viability, locomotor scores and tissue or molecular measures versus injury controls.

Results-extraction completeness

The full article was checked for methods, intervention details, outcomes, positive and null findings, funding and conflicts. This record does not imply medical efficacy.

A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.

Interpretation limits

Cautions and applicability

Design and reporting cautions

Animal and cell evidence only; the combination design cannot isolate all effects of hydrogen.

Applies directly to

The reported rat spinal-injury and cell models only.

Preliminary appraisal framework

SYRCLE animal-study tool — preliminary

Preliminary risk-of-bias status

Some concerns

Appraisal rationale

Controls were present, but concealment, assessor blinding and selective-outcome risk were unclear.

Appraisal domains

Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified

No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.

Provenance

Sources and record status

Identifiers

PMID: 37181828 · DOI: 10.1155/2023/8227382

Publisher access

A free full article is available through PubMed Central.

Extraction basis

Official PubMed Central full article and PubMed bibliographic record; source identity, methods, intervention, results, funding and conflicts checked 10 August 2026.

Record revision

2026-08-10