Hydrogen-rich alkaline water supplementation restores a healthy state and redox balance in H₂O₂-treated mice
Mizzoni D, Logozzi M, Di Raimo R, Spada M, Fais S. · Int J Mol Sci. 2024;25(12):6736.
Study at a glance
Preclinical
Female mice after hydrogen-peroxide exposure.
Commercial hydrogen-rich alkaline water after two weeks of 1% hydrogen peroxide. · Product claimed approximately 1 ppm H₂, pH 9 and 1 g/L formulation.
Three weeks of product water after the oxidative challenge.
Several oxidative and cellular measures favored the product-water group after hydrogen-peroxide exposure.
The alkaline water contained minerals and vitamin C, so effects cannot be assigned specifically to molecular H₂.
What kind of evidence is this?
Preclinical
Controlled mouse study
Other molecular hydrogen research
Other forms
Positive biomarker signal for a complex water product.
Systemic and cellular biomarker outcomes in mice.
Methods
Female mice after hydrogen-peroxide exposure.
30 mice; three groups of 10.
Three weeks of product water after the oxidative challenge.
Commercial hydrogen-rich alkaline water after two weeks of 1% hydrogen peroxide.
H₂ dissolved in an alkaline, mineral-containing water product.
Product claimed approximately 1 ppm H₂, pH 9 and 1 g/L formulation.
Untreated water and hydrogen-peroxide groups.
Outcomes and reported result
Plasma, spleen and marrow oxidative markers, mitochondrial measures and cell proliferation.
Several oxidative and cellular measures favored the product-water group after hydrogen-peroxide exposure.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
The alkaline water contained minerals and vitamin C, so effects cannot be assigned specifically to molecular H₂.
The tested product and mouse model only.
SYRCLE animal-study tool — preliminary
High risk
Complex intervention, unclear allocation and blinding and multiple biomarker outcomes.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 38928440 · DOI: 10.3390/ijms25126736
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
10 August 2026
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