Hydrogen-rich alkaline water supplementation restores a healthy state and redox balance in H₂O₂-treated mice
Mizzoni D, Logozzi M, Di Raimo R, Spada M, Fais S. · Int J Mol Sci. 2024;25(12):6736.
What kind of evidence is this?
Preclinical
Controlled mouse study
Other molecular hydrogen research
Other forms
Positive biomarker signal for a complex water product.
Systemic and cellular biomarker outcomes in mice.
Methods at a glance
Female mice after hydrogen-peroxide exposure.
30 mice; three groups of 10.
Three weeks of product water after the oxidative challenge.
Commercial hydrogen-rich alkaline water after two weeks of 1% hydrogen peroxide.
H₂ dissolved in an alkaline, mineral-containing water product.
Product claimed approximately 1 ppm H₂, pH 9 and 1 g/L formulation.
Not applicable.
No oxygen was co-delivered.
Untreated water and hydrogen-peroxide groups.
Outcomes and reported result
Plasma, spleen and marrow oxidative markers, mitochondrial measures and cell proliferation.
Several oxidative and cellular measures favored the product-water group after hydrogen-peroxide exposure.
The full article methods, intervention, outcomes, positive and null findings, funding and conflict statements were checked.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
The alkaline water contained minerals and vitamin C, so effects cannot be assigned specifically to molecular H₂.
The tested product and mouse model only.
SYRCLE animal-study tool — preliminary
High risk
Complex intervention, unclear allocation and blinding and multiple biomarker outcomes.
Sequence generation checked where reported · Allocation concealment generally unclear · Personnel blinding generally unclear · Outcome-assessor blinding generally unclear · No prospectively registered analysis plan identified
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 38928440 · DOI: 10.3390/ijms25126736
A free publisher or repository full article was checked.
Publisher or repository full article and bibliographic record; checked 10 August 2026.
2026-08-10