Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis
He J, Xu P, Xu T, Yu H, Wang L, Chen R, Zhang K, Yao Y, Xie Y, Yang Q, Wu W, Sun D, Wu D. · Frontiers in aging neuroscience. 2024;16:1515092.
Study at a glance
Preclinical
Adult zebrafish in an aluminum-chloride Alzheimer-like model, with separate zebrafish-larva assays.
Adult HRW group: aquarium water at 1,000 ppb dissolved H₂ for 10 hours/day, followed by 14 hours/day in the normal environment, for 7 days. Separate larval experiment: 1,000 ppb HRW for 3 days after 4 days of Al³⁺ exposure; medium was renewed daily. · Dissolved H₂ target: 1,000 ppb (approximately 1 mg/L). The reported 20-minute period was the water-preparation time needed to reach the target concentration, not the zebrafish exposure duration.
Adults: 10 hours/day for 7 days. Larvae: 3 days in a separate experiment.
The article reports less amyloid-β deposition, lower inflammatory and oxidative-stress measures, altered sEH/EET measures and partial behavioral and microbiota improvements in HRW-treated zebrafish versus the Alzheimer-model group. This is preclinical evidence only.
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
What kind of evidence is this?
Preclinical
Controlled preclinical study
Other neurological conditions
H₂-rich water
Information not yet classified
Some editorial classification fields are still pending. The source-reported outcomes and result are shown below; Hydrogenology does not infer a positive or negative signal from prose automatically.
Methods
Adult zebrafish in an aluminum-chloride Alzheimer-like model, with separate zebrafish-larva assays.
Adult experiment: three groups of 120 zebrafish each. Separate larval and assay-level analyses used smaller source-reported subsamples.
Adults: 10 hours/day for 7 days. Larvae: 3 days in a separate experiment.
Adult HRW group: aquarium water at 1,000 ppb dissolved H₂ for 10 hours/day, followed by 14 hours/day in the normal environment, for 7 days. Separate larval experiment: 1,000 ppb HRW for 3 days after 4 days of Al³⁺ exposure; medium was renewed daily.
H₂ dissolved in drinking water — H₂ only unless the full text explicitly reports oxygen co-delivery.
Dissolved H₂ target: 1,000 ppb (approximately 1 mg/L). The reported 20-minute period was the water-preparation time needed to reach the target concentration, not the zebrafish exposure duration.
Adult control and Alzheimer-model groups remained in normal water; the larval experiment used corresponding control and aluminum-exposed conditions.
Outcomes and reported result
Behavior and cognition, amyloid-β deposition, neuroinflammation, oxidative-stress markers, liver sEH/EET measures and gut microbiota.
The article reports less amyloid-β deposition, lower inflammatory and oxidative-stress measures, altered sEH/EET measures and partial behavioral and microbiota improvements in HRW-treated zebrafish versus the Alzheimer-model group. This is preclinical evidence only.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit.
Limitations and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. Funding or grant information is reported in the full article. The article declares no conflicts of interest.
Applies to the reported animal, cell or tissue model, not directly to patients.
No single-study GRADE certainty rating is assigned. Read how records and evidence assessments are prepared.
Sources and record status
PMID: 39839307 · DOI: 10.3389/fnagi.2024.1515092
A free full article is available through PubMed Central.
Official PubMed/PMC full article; record re-audited and corrected 25 August 2026.
25 August 2026
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