Pharmacokinetics of Hydrogen During Hydrogen-Saturated Saline Infusion in Pigs
Shibuya M, Fujinaka M, Yonezawa M, Nishimura N, Uchinoumi H, Sunahara H, Tani K, Kobayashi E, Sano M. · Biomedicines. 2025;13(1):234.
What kind of evidence is this?
Preclinical
Controlled preclinical study
Cardiovascular and circulatory health
Other forms
Not reported in this record.
Not reported in this record.
Methods at a glance
pigs, pig used to study cardiovascular and circulatory health.
Preclinical study; the article does not state one consolidated sample total, so no total is inferred.
120 min
Full text reports an injected or infused H₂ intervention. Detected intervention quantities or schedules: 1 mL, 3 mL/kg, 120 min, 30 min, 60 min, 90 min, 500 mL, 8 weeks.
an injected or infused H₂ intervention — H₂ only unless the full text explicitly reports oxygen co-delivery.
H₂-related quantities reported in the intervention passages: 1 mL, 3 mL/kg, 120 min, 30 min, 60 min, 90 min, 500 mL, 8 weeks. Values are not combined across different experimental arms.
Not applicable — no inhaled H₂ intervention was identified in the full article.
No inhaled O₂ co-delivery was identified for this non-inhalation intervention.
Control or comparison condition reported in the full article; see the linked methods for arm-specific details.
Outcomes and reported result
clinical symptoms or functional scores, tumor growth or cancer markers, inflammation and cytokines, apoptosis or cell injury, histology or tissue damage, metabolic laboratory measures
Article-reported result excerpt (24 words maximum): “The analysis of the hydrogen-saturated saline showed that the color of the solution was clear and colorless, the pH was 5.6, and the sodium…” This excerpt is not a complete result summary; consult the linked full text for all positive and null findings.
The complete machine-readable article was checked for source identity, methods, intervention quantities, results, tables, funding and conflict declarations. This public record is based on the full article.
A reported association, difference or mechanism is not automatically a clinical benefit. Null findings, outcome type, study design and precision all matter.
Cautions and applicability
Preclinical evidence only; findings do not establish a treatment effect in people. No funding statement was identified in the machine-readable full article; absence is not inferred. No explicit conflict statement was identified in the machine-readable full article; absence is not inferred.
Applies to the reported animal, cell or tissue model, not directly to patients.
Not reported in this record.
Not reported in this record.
Not reported in this record.
No single-study GRADE certainty rating is assigned. Certainty is assessed by important outcome across a complete eligible evidence set, not by attaching a final grade to one source.
Sources and record status
PMID: 39857817 · DOI: 10.3390/biomedicines13010234
A free full article is available through PubMed Central.
Official Europe PMC JATS full article and PubMed/PMC identifiers; structured full-text extraction and validation completed 8 August 2026.
2026-08-10